中药单体丹参酮Ⅱa联合丝裂霉素对人肝癌细胞株HepG2体外作用实验研究.pdf

目 录 中英文缩略词 ···········································1 中文摘要 ················································3 英文摘要 ················································7 引 言 ···············································10 正 文 ···············································13 1.实验材料和方法 ·····································13 1.1 实验材料 ······································13 1.2 细胞培养、冻存及复苏···························15 1.3 相差显微镜观察药物对HepG2 细胞形态的影响 ········18 1.4 MTT(噻唑蓝)比色实验·····························18 1.5 流式细胞术检