甘草水提液对吸烟小鼠肺组织超氧化物歧化酶和丙二醛的影响_药学论文.docVIP

甘草水提液对吸烟小鼠肺组织超氧化物歧化酶和丙二醛的影响_药学论文 甘草水提液对吸烟小鼠肺组织超氧化物歧化酶和丙二醛的影响_药学论文 【摘要】 目的观察甘草水提液对吸烟小鼠肺组织超氧化物歧化酶（SOD）和丙二醛（MDA）的影响。方法昆明种小鼠，随机分成3组，即正常对照组、吸烟模型组、甘草水提液组。正常对照组小鼠不吸烟，其余两组连续吸烟60 d，4次/d，30 min/次，甘草水提液组2次/d给予蒸馏水灌胃对照，60 d后处死动物，取左上肺固定，石蜡包埋，切片HE染色观察，其余肺组织做成匀浆，检测肺组织匀浆中SOD活性，MDA含量。结果吸烟组小鼠肺内出现程序不等的慢性支气管炎及肺气肿，同时肺部SOD活性明显下降，MDA含量明显增加。吸烟后加服甘草水提液组小鼠肺部病理表现明显减轻，肺部的SOD活性增加，MDA含量下降。结论 甘草水提液对小鼠长期吸烟所造成的支气管肺组织病理损害有保护作用，其作用机制可能与甘草在肺部发挥的抗氧化作用等有关。 【关键词】 甘草水提液； 吸烟； 肺组织； 超氧化物歧化酶； 丙二醛 　　The Protective Effects of Glyrrhizae on Smoking induced Pulmonary Injury in Mice 　　Abstract:ObjectiveTo investigate the protective effects of glycyrrhizae water-extraction on smoking-induced pulmonary injury in mice.MethodsKuming mice were randomly divided into 3 groups：healthy control group,model group and glycyrrhizae group. Except healthy control group,the others were exposed to cigarette smoke,qid,30 minute/day for 60 days. At the same time,the mouse of glycyrrhizae group were given glycyrrhizae water-extracion,bid for 60 days,the other mouse were given distilled water,bid for 60 days.The pathologic changes of airways and lung tissues were determined.The level of MDA and the activity of SOD were measured.ResultsThere were more chronic bronchites and emphysema in the model group than in the healthy control group. Cigarette smoking reduced the activity of SOD and increased MDA level.Compared with the model group,the pathologic injury of airways and lung tissues were reduced,the activity of SOD increased and the level of MDA decreased in glycyrrhizae group.ConclusionGlyrrhizae water-extract has the protective effect on smoking- induced injury.The mechanism may due to its antioxidation. 　　Key words:Glycyrrhizae water-extraction; Smoking； Pulmonary injury; Superoxide dismutase； Malodialdenhyde 　　吸烟有害健康。众多资料表明，吸烟可致慢性呼吸道炎症，如咽炎、气管及支气管炎、支气管扩张及肺气肿等症，并可诱发肺癌及其它癌肿。由于香烟中有多种有害成分，如CO、尼古丁、焦油、苯丙芘、亚硝胺等，因此，吸烟所致的损害是一种多系统的病理变化［1］。为了预防吸烟导致的慢性呼吸道炎症，延缓咽炎、支气管炎等发病时间，寻找能预防吸烟所致的肺损伤的中药，笔者建立了小鼠主动吸烟的动物模型，筛选和研究了一些中药对这些动物模型的预防作用。现报道如下。 　　1 材料