小麦优质谷蛋白亚基分子标记多重PCR体系的建立与应用.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2009, 35(10): 1831−1835 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2009.01831 小麦优质谷蛋白亚基分子标记多重PCR 体系的建立与应用 郑 寒1,2 陈 静1,* 任 妍1,2 余懋群1 付体华2 1 , 610041; 2 , 625014 * OE : Ax1/Ax2 Dx5 Bx7 (Bx7 ) PCR , , PCR 89 (), , Dx5 Ax1/Ax2* 12.4%, Bx7OE, 10.1%, Ax1/Ax2*Dx5 Bx7OE PCR , : ; (HMW-GS); PCR Establishment and Application of Multiplex-PCR for Wheat Glutenin Subunits Relative to Superior End-Use Quality 1,2 1,* 1,2 1 2 ZHENG Han , CHEN Jing , REN Yan , YU Mao-Qun , and FU Ti-Hua 1 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; 2 College of Agronomy, Sichuan Agricultural University, Ya’an 625014, China Abstract: Wheat processing qualities are largely dependent on the number and composition of high-molecular-weight glutenin subunits (HMW-GS). It has been demonstrated that Ax1/Ax2*, Dx5, and overexpressed Bx7 (Bx7OE) are normally associated with superior end-use quality, especially dough strength. To date, Bx7OE allele has not been exploited widely in China wheat breeding program. With the optimization of reaction components and amplification condition, three pairs of PCR primers targeting for Ax1/Ax2*, Bx7OE, and Dx5 genes were used to establish a multiplex PCR for the purpose of enhanced molecular marker-assisted breeding with good reliability and low cost. The validation with wheat cultivars (line