不同产地_不同年限人参中淀粉酶_酯酶_酸性磷酸酯酶的活力比较.pdfVIP

·专 栏 · ·中药与天然药 · 不同产地、不同年限人参中淀粉酶、酯酶、酸性磷酸酯酶的活力比较 邢楠楠 1 1* 1 1 2 ，赵雨 ，刘宏 ，张惠 ，李红艳 (1.长春中医药大学，长春 130117；2.辽宁中医药大学大连校区，辽宁 大连 116600) 摘要：目的 对不同产地、不同生长年限人参进行淀粉酶(AMY)、酯酶(Est)和酸性磷酸酯酶(ACP)活力比较。方法 用中 性缓冲溶液提取总蛋白，应用 3,5-二硝基水杨酸比色法测定 AMY 活力；乙酸-α-萘酯比色法测定 Est 活力；对硝基酚比色 法测定 ACP 活力。结果 不同产地人参 AMY 、Est 、ACP 活力均存在很大差异。取不同产地、相同生长年限酶活力平均 值进行不同年限的酶活力比较，可知 4 年与 5 年生人参 3 种水解酶活力均无明显差异。结论 AMY 、Est 、ACP 活力可 以作为评价人参质量的内在指标之一。 关键词：人参；淀粉酶；酯酶；酸性磷酸酯酶 中图分类号：R284.1 文献标志码：B 文章编号：1007-7693(2011)01-0044-04 Comparison of Activities of Amylase, Esterase, Acid Phosphatase in Ginseng Radix et Rhizoma from Different Origins and Different Growth Periods 1 1* 1 1 2 XING Nannan , ZHAO Yu , LIU Hong , ZHANG Hui , LI Hongyan (1.Changchun University of Chinese Medicine, Changchun 130117, China; 2.Liaoning University of Traditional Chinese Medicine, Dalian Campus, Dalian 116600, China) ABSTRACT ：OBJECTIVE To compare activities of Ginseng Radix et Rhizoma amylase(AMY), esterase (Est) and acid phosphatase (ACP) from different origins and different growth periods. METHODS Total protein was extracted using a neutral buffer solution. Determination of AMY activity applied 3,5-dinitrosalicylic acid colorimetry. Determination of Est activity was using acetic acid-α-naphthyl ester colorimetry. Determination of ACP activity was using p -nitrophenol colorimetry. RESULTS There was a significant difference in activities of AMY, Est and ACP in Ginseng Radix et Rhizoma from different origins. Take the average value for enzyme activity in Ginseng Radix et Rhizoma of different origin and the same growth period, purpose was to compare the enzyme activity at different ages. We can know that activities of three k