不同培养条件对红曲霉产红曲色素的研究.pdfVIP

※生物工程 食品科学 2008, Vol. 29, No. 01 215 不同培养条件对红曲霉产红曲色素的研究 郭红珍，王秋芬，马立芝 (廊坊师范学院生命科学学院，河北 廊坊 065000) 摘 要：本研究主要针对发酵液培养基中不同碳源、氮源、pH 以及装液量分别进行单因素五水平的试验，确定 三个较好的水平，再进行四因素三水平正交试验以确定红曲霉产红曲色素的最佳条件。在单因素试验中，碳源以 玉米粉、淀粉、麦芽糖较好；氮源以硝酸钠、大豆粉、氯化铵较好；pH 值以pH3.8、5.4、7.0 较好；装液 量以250ml三角瓶装100、125、150ml 较好。通过四因素三水平正交试验，确定出红曲霉产红曲色素的最佳培养 条件为A3B1C1D3 ，即麦芽糖、大豆粉、pH3.8、装液量 150ml/250ml 组合最佳。 关键词：液态发酵；红曲霉；红曲色素 Study on Liquid Fermentation of Monascus ruber Pigment at Different Culture Conditions GUO Hong-zhen，WANG Qiu-fen，MA Li-zhi (Department of Biology, Langfang Teachers College, Langfang 065000, China) Abstract ：With carbon, nitrogen, pH value and fermentation liquid volume in flask as test factors, producing conditions of pigment by 4 Monascus rubber were optimized by L9 (3) orthogonal test. Signal test results showed that corn powder, maltose and starch are the better carbons; NaNO3, soya powder, NH4Cl are the better nitrogen; pH3.8, pH5.4, pH7.0 are the better pH values of substrate; and 100 ml/250 ml, 125 ml/250 ml, 150 ml/250 ml are the better fermentation liquid volumes. Orthogonal test results showed that A3B1C1D3 is the optimum conditions combination for producing Monascus rubber pigments, that is to say, maltose as carbon, soya powder as nitrogen, pH3.8 and 150 ml/250 ml fermentation liquid volume in flask are the optimum conditions. Monascus rubber；monascus pigment Key words：liquid fermentation； 中图分类号：TS264.4 文献标识码：A 文章编号：1002-6630(2008)01-0215-04 红曲霉以能产生大量红曲色素而得名。红曲自古以 由于红曲霉生产红曲色素受到碳源、氮源、p H 来在我国一直用于食品的着色，特别是肉制品的着色[1]。 值、装液量等多种因素的影响