基于生物反应器制药废水中活的但非可培养状态细菌的分离.pdfVIP

Agricultural Science Technology, 2014, 15(8): 1299-1303 Copyright 訫 2014, Information Institute of HAAS. All rights reserved Molecular Biology and Tissue Culture Viable but Non鄄culturable Bacteria in Bioreactor鄄 based Pharmaceutical Wastewater Yanyan ZOU1, Huiling FU1, Yahong CHEN2, Jingyu ZHAI1, Yanfei WEI3, Rongwu MEI3, Linxian DING1 鄢 1. College of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua 321004, China; 2. Zhejiang Jinhua Conbe Bio-Pharm Co., Ltd. Jinhua 321016, China; 3. Environmental Science Research Design Institute of Zhejiang Province, Hangzhou 310007, China Abstract [Objective] We aimed to investigate the composition and phylogenetic rela鄄 基于 生物反应器制药废水中活 tionships of the viable but non鄄culturable (VBNC) state bacteria in pharmaceutical 的但非可培养状态细菌的分离 wastewater. [Method] Soil filter was used for constructing bioreactor. Based on the resuscitation鄄 and growth鄄promoting function of Resuscitation Promoting Factor (Rpf) 邹艳艳 1，付 惠玲 1，陈雅红 2 ，翟竟余 1，韦彦斐 3 ， for VBNC bacteria, VBNC bacteria were isolated by most probable number (MPN) 梅 荣武 3 ，丁林贤 1* （1.浙 江师 范大学 地理 与 method and dilution鄄plating method and 16S rRNA gene phylogenetic analysis was carried out. [Result] In MPN culture system, Rpf could promote the resuscitation and 环境科学学院 ， 浙江金华 321004 ；2. 浙 江金 growth of some bacteria. There were VBNC advantage floras that sensitive to Rpf 华 康 恩 贝 生 物 制 药 有 限 公 司 ， 浙 江 金 华 in pharmaceutical wastewater. The culturable VBNC bacteria in pharmaceutical 321016 ；3. 浙 江省环境保护科学设计研究院 ， wastewater consisted of high鄄GC gram鄄positive actinomycetes including genera Mi鄄 浙江杭州 310007） crobacterium, Gordonia and Leucobacter, and gram鄄negative bacteria including gen鄄