单位面积内细胞数的计算方法.pdfVIP

Nephrol Dial Transplant (2006) 21: 624–633 doi:10.1093/ndt/gfi225 Advance Access publication 9 January 2006 Original Article Kallikrein/kinin protects against gentamicin-induced nephrotoxicity by inhibition of inflammation and apoptosis Grant Bledsoe, Sarah Crickman, Jenny Mao, Chun-Fang Xia, Hideyuki Murakami, Lee Chao and Julie Chao Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425-2211, USA Abstract Keywords: apoptosis; gentamicin; inflammation; Background. Our previous study showed that kalli- kidney; oxidative stress krein gene transfer protects against gentamicin- induced nephrotoxicity and enhances renal function. In this study, we investigated the effects and potential mechanisms of kallikrein/kinin on inflammation and Introduction apoptosis induced by gentamicin. Methods. Rats were injected subcutaneously with Gentamicin is an aminoglycoside antibiotic used in gentamicin daily for 10 days and received an intra- the treatment of Gram-negative bacterial infections. venous injection of adenovirus carrying the human As gentamicin administration can induce severe tissue kallikrein gene or control virus on the first day of nephrotoxicity [1], it has become a popular substance gentamicin administration. used to study drug-induced acute renal failure. Results. After 10 days of gentamicin treatment, Thus, a therapeutic approach to protect or reverse kallikrein gene transfer significantly attenuated aminoglycoside-induced kidney injury would have gentamicin-induced tubular dilatation and lumenal signific