银杏叶提取物诱导大鼠主动脉平滑肌细胞中血红素氧合酶-1表达的实验研究_药学论文.docVIP

银杏叶提取物诱导大鼠主动脉平滑肌细胞中血红素氧合酶-1表达的实验研究_药学论文 银杏叶提取物诱导大鼠主动脉平滑肌细胞中血红素氧合酶-1表达的实验研究_药学论文 作者：汪红霞，杨晓瑜，魏宗德，黄维义 【摘要】 　　目的探讨银杏叶提取物（EGb）诱导大鼠主动脉平滑肌细胞（SMC）中血红素氧合酶-1(HO-1)表达的量效关系，并初步探讨EGb诱导HO-1表达的分子机制。方法复苏并传代培养大鼠主动脉SMC，分组实验，分别采用0，50，100，200，400 μg/ml 5个不同浓度的EGb以及200 μg/ml EGb加放线菌素D（ActD）孵育SMC 8 h，Western blot法检测HO-1蛋白，以HO-1条带灰度值代表HO-1的表达量。结果不加EGb的对照组中HO-1蛋白仅有微量基础表达，加用EGb各组中HO-1蛋白的表达随EGb浓度的增加而增加（P＜0.05），在EGb浓度为200 μg/ml时达到峰值，随后则有所下降。加用ActD能显著抑制EGb所诱导的HO-1蛋白表达（P＜0.05）。结论EGb能够诱导大鼠主动脉SMC中HO-1表达，且这种表达在一定范围内具有量效关系；EGb诱导HO-1表达有赖于基因重新转录。 【关键词】 银杏叶提取物 大鼠 主动脉平滑肌细胞 血红素氧合酶-1 　　Abstract：ObjectiveTo explore the dose-effect relationship and the molecular mechanism of the heme oxygenase-1(HO-1) expression induced by the extract of Gingko biloba(EGb) in rat aorta smooth muscle cells (SMC).MethodsThe SMC was revived,passage cultivated and divided into different groups.The cells in each group were incubated with 5 concentrations of EGb such as 0，50，100，200，400 μg/ml and EGb 200 μg/ml+ActD4 μm respectively. The incubation lasted for 8 hours,then Western blot was applied to analyze HO-1 protein expression.ResultsThere was only basal microamount expression in the group without EGb. EGb significantly induced the expression of HO-1 in SMC and the expression increased with the increase of EGb concentration (P＜0.05),it reached the peak amplitude under the condition of EGb 200 μg/ml,then desceded.EGb-induced HO-1 expression was significantly inhibited by ActD.ConclusionEGb can induce HO-1 expression in rat aorta SMC and the expression has a dose-dependent feature under a limited EGb concentration range.EGb-induce HO-1 protein expression depends on HO-1 genetic retranscription. 　　Key words：Extract of gingko biloba； Rat; Smooth muscle cell; Heme oxygenase-1 　　动脉粥样硬化（atherosclerosis,AS）是危害人类健康的严重心血管疾病，近年来其发病率和死亡率更是不断上升，对其防治方法的研究一直倍受关注。已知氧化应激损伤是致AS的核心环节，抗氧化应激已成为防治AS的有效途径之一。血红素氧合酶-1（Heme oxygenase-1,HO-1）是血红素氧合酶的诱导型，在应对氧化应激损伤中发挥着不可或缺的作用，逐渐积累的证据显示，HO-1系统在抗AS中起着重要作用［1］。银杏叶提取物（Extract of Gingko biloba,EGb）是传统中药银杏中的一种