食管鳞癌多阶段癌变中14 3 3σ和P53表达特征及相关性分析_医学论文.docVIP

食管鳞癌多阶段癌变中14 3 3σ和P53表达特征及相关性分析_医学论文 食管鳞癌多阶段癌变中14 3 3σ和P53表达特征及相关性分析_医学论文 作者：齐义军，马远方，杜耀武，刘广超，张蕾蕾，张国民，施巩宁，张双林，李勇，何占锋，何庆瑜 【摘要】 　　目的： 探讨14和P53蛋白表达在食管鳞癌和癌旁正常黏膜上皮、各级癌前病变中的变化特征及相关性. 方法： 采用免疫组织化学方法检测14和P53蛋白在60例不同分化程度食管鳞癌和癌旁正常食管黏膜上皮、各级癌前病变中的表达变化. 结果： 癌旁正常食管黏膜上皮中均可检测到不同程度的14蛋白表达（表达量8.22），随着癌前病变进展，14的表达率和表达量逐渐下降；低分化鳞癌中14的表达量最低（表达量0.45），完全失表达率为64%（7/11）. P53蛋白在鳞癌中表达率最高67%（27/40），表达量为4.86，而只有25%（6/24）癌旁正常上皮表达P53蛋白（表达量0.42）. 14和P53蛋白的相关性分析表明二者呈显著负相关（0.05）. 结论： P53蛋白可能通过负调控14基因的表达参与食管癌变多阶段演进过程，14和P53可能成为食管癌早期诊断及高危人群筛查的分子指标，同时也可能成为食管癌生物预防和疗效评价的新靶点. 【关键词】 食管肿瘤；癌，鳞状细胞；14；基因，P53；分子标记物 　　【Abstract】AIM: To characterize the 14s epithelium. METHODS: Immunohistochemistry (ABC) methods were used to determine 1433σ and P53 protein expressions in 60 cases of ESCC, nearby matched normal esophageal epithelium and a variety of ESCC precursors. RESULTS: High expression of 1433σ was found ubiquitously in normal esophageal epithelium with a digitalized average value of 8.22 in expression. Protein 1433σ was downregulated stepwise during the development of esophageal carcinoma from normal epithelium. Sixtyfour percent of poorlydifferentiated squamous cancer lost 1433σ expression with the digitalized value of 0.45. However, pattern of P53 protein expression was in contrast to 1433σ. Sixtyseven percent of esophageal cancer expressed P53 at a high level with a quantitated amount of 4.8. In nearby normal epithelium of esophagus, the P53 protein expression was low with a digitalized value of 0.42. Significant negative correlation between 1433σ and p53 expressions was revealed by Spearman rank correlation analysis (0.05). CONCLUSION: The aberrant expression pattern of 1433σ and p53 may contribute to the formation and progression of ESCC and may predict the prognosis of ESCC patients. Proteins 1433σ and P53 have the potential to become biomarkers for early diagnosis of esophageal cancer and endpoint molecules of ESCC prevention. 　　【Keywords】 esophageal neoplasms carcinoma, squamou