牛血清白蛋白色谱柱的制备及对阿司匹林的含量测定.pdfVIP

中国医药导报 2014年6月第 11卷第 16期 窑药品检验 窑 牛血清白蛋白色谱柱的制备 及对阿司匹林的含量测定 唐玲利1 韦室百2 黄东萍1 黄明立1 阮 冲1 1.广西医科大学公共卫生学院袁广西南宁 530021曰2.广西医科大学第一附属医院药学部袁广西南宁 530021 [摘要] 目的制备生物色谱柱袁用于分离或测定药物成分遥 方法 采用3-氨基丙基三甲氧基硅烷渊APTS冤与硅胶 反应生成氨基功能化的活化硅胶袁并与牛血清蛋白渊BSA冤共价结合形成BSA键合固定相袁匀浆装柱制备成BSA 生物色谱柱袁以阿司匹林渊Aspirin袁ASP冤为分析对象考察BSA 固定相的结合性能和分离性能遥 结果 BSA结合率 为3.69 滋mol/g袁ASP在BSA键合固定相色谱柱上的最大结合量为74 mg/g袁经BSA色谱柱测得阿司匹林肠溶片 的含量为14.65滋g/片袁标示量为 107.8%遥 结论这表明BSA与硅胶载体成功结合形成BSA键合固定相袁所制备 的BSA生物色谱柱具有良好的结合性能与分离性能遥 [关键词] 牛血清白蛋白曰生物色谱柱曰阿司匹林 [中图分类号] R96 [文献标识码] B [文章编号] 1673-7210渊2014冤06渊a冤-0101-04 Preparation of bovine serum albumin biological chromatographic column anditsapplicationin determinationofAspirin 1 2 1 1 1 TANGLingli WEIShibai HUANG Dongping HUANG Mingli RUAN Chong 1.College of Public Health of Guangxi Medical University, Guangxi Zhuang Autonomous Region, Nanning 530021, China; 2.Department of Pharmacy, the First Affiliated Hospital of Guangxi Medical University, Guangxi Zhuang Au鄄 tonomous Region,Nanning 530021, China [Abstract] Objective To prepare biological chromatographic column to separate and determine the ingredients of drugs.Methods Silicagelwas reacted with 3-aminopropyltrimethoxylsilane toform aminefunctionalized activated sil鄄 ica gel, which was covalent binding with BSA to synthesize BSA bonded stationary phase. Then BSA bonded stationary phasewas packed columnwith homogenization,thus BSAbiological chromatographic columnwas prepared. Aspirin was taken as a detective object to examine the combination performance and separation performance of BSA bonded sta鄄 tionary phase. Results It came to that, the combined rate was 3.69 滋mol/g, the biological chromatographic column was 74 mg/g, the amou