左旋多巴甲酯对剥夺性弱视猫的作用.pdfVIP

左旋多巴甲酯对剥夺性弱视猫的作用 黎荣梁韬林兴张士军李勇文黄仁彬· 广西医科大学药学院，广西南宁530021 摘要 目的：观察左旋多巴甲酯对剥夺性弱视猫模型视皮质17区神经细胞凋亡情况以及c—fos表达的作 用，探讨左旋多巴甲酯治疗弱视的机制。方法：健康的幼猫随机分组：正常对照组 NC 、模型对照组 MC 实验方法对幼猫进行左眼单纯上下睑缝合诱导剥夺性弱视，12周后打开缝合眼并开始给药，每天灌胃左旋 区神经细胞中c—fos的表达情况。结果：与模型对照组比较，给药组视皮质17区中的细胞凋亡数量有明 显降低，而c-fosmRNA则显著的上调 只0．01 。结论：左旋多巴甲酯对弱视诱发的视皮质17区中神经 细胞有抗凋亡作用以及增加内源性c—fos的表达来改善剥夺性弱视的功能障碍。 关键词：左旋多巴甲酯；剥夺性弱视；细胞凋亡：c—fos；视皮质17区 基金项目：国家自然科学基金项目 项目号：座机电话号码 ，广西2011年研究生教育创新计划资助项目 No 1iron91278@163．tom E-mai l：huangrenbin518@163．com of esteronvisualcortex17areaofmonocular Effects L-dopamethyl cats deprivation LI Ren—bin* Tao：LINXing：ZHANGShi—jun：LIYong—wen：HUANG Rong：LIANG SchoolofPharmaceutical Medical 530021，China Sciences，GuangxiUniversityNanning ofthe the observetheeffects methylester LDME onexpression [Abstract]Objective：To L-dopa in ofthevisualcortexofcatswithmonocular ofTUNEL cellS，c-fos neurons positive expression its the effectofLDMEon and deprivation，andexploretherapeutic amblyopia Thenormalcatswere dividedinto catsineach randomly groups 5 group ：low doseof control dose LDMEM ，andhigh L—dopamethylester LDME, ，positive control modelcontrol totheclassic ofHubel group NC and group MC ．Referringexperiments in and thelidsof …．thekittenseach wereanesthetizedvisuallydeprivedbysuturing group closurewas andtreat theleft to themonocular amblyopia，theneye open eyesperform deprivation with after12weeks thenormalcontrolwas methyl drug groupexcluded ．L—dopaester 20mg／kg， normalsalinewere tothe 40mg／kg，80mg／kg ，L—dopa 40mg／kg and givenrespectively animalsfor30 withTUNEL wererecordedtocheckthe corresponding days．TheapoptosiS staining wasusedtodetectthe ofC—fos cats’visumcortexfeatures．TheRT—PCR expression technique control treatmentshowed tothemodel groups significantly mRNA．Results：Comparing group，the ofTUNEL cellS．reducednumberofC—fosmRNA decreased positive level ，K0．01 ． cytometriCanalysiS Conclusion：Theresults that ester suggestLevodopamethyl mayprevent andmakearestorationofvisualfunctionalde