助孕胶囊对多囊卵巢大鼠血清性激素水平及卵巢组织P450arom表达的影响_临床医学论文.docVIP

助孕胶囊对多囊卵巢大鼠血清性激素水平及卵巢组织P450arom表达的影响_临床医学论文 助孕胶囊对多囊卵巢大鼠血清性激素水平及卵巢组织P450arom表达的影响_临床医学论文 【摘要】 目的 观察助孕胶囊对多囊卵巢(PCO)大鼠卵巢形态学、血清性激素水平及卵巢P450arom表达的影响,探讨其对PCO大鼠可能的作用靶点和机制。方法 采用胰岛素联合人绒毛膜促性腺激素(HCG)造模法制备PCO模型大鼠,随机分为对照组、模型组、助孕胶囊组、二甲双胍组,按成人剂量的30倍给药。助孕胶囊组给予3.0 g/(kg·d),二甲双胍组为0.75 g/(kg·d),对照组和模型组大鼠灌胃等体积蒸馏水1 mL/100 g体重。光镜观察卵巢、卵泡发育情况,放免法测定血清性激素水平,免疫组化法检测卵巢组织P450arom的表达水平。结果 助孕胶囊可使PCO大鼠卵巢内颗粒细胞层数增加,泡膜细胞层变薄；血清睾酮、黄体生成素、胰岛素水平下降,卵泡刺激素、雌二醇水平升高；并上调卵巢组织颗粒细胞中P450arom的表达水平。结论 助孕胶囊可通过直接或间接作用,影响卵巢组织P450arom的表达,从而发挥调整PCO大鼠生殖内分泌作用,促进卵泡的发育成熟及排卵。 【关键词】 多囊卵巢综合征；助孕胶囊；性激素；P450arom；大鼠 Abstract：Objective To observe the effects of Zhuyunjiaonang on morphological change of the ovary, serous hormone and the expression of P450arom in PCO model rats. To explore its target and mechanism in treating PCO model rats. Methods Using INS and HCG to establish PCO model. The PCO rat models were devided into 4 groups randomly, which included control group, model group, Zhuyunjiaonang group and metformin group. Zhuyunjiaonang group was given 3.0 g/(kg·d) Zhuyunjiaonang, metformin group was given 0.75 g/(kg·d) metformin, control group and model group were given same volume distilled water 1 mL/100 g. The effects of Zhuyunjiaonang on ovaries and ovarial follicular development were observed by light microscope. The levels of serum gonadal hormone were detected by radioimmunoassay. The expression level of P450arom was detected with immunohistochemical staining technique. Results Zhuyunjiaonang could increase the thickness of granular cell layer and decrease the thickness of theca cell layer, decrease the level of T and LH, and increase the level of FSH and E2 in serum. Meanwhile the expression of P450arom in ovary were increased. Conclusion Zhuyunjiaonang could indirectly or directly affect the expression of the P450arom in ovary, it could regulate the endocrine function of PCO rat and promote the mature and ovulation of follicular. Key words：polycystic ovarian syndrome；Zhuyunjiaonang；gonadal hormone；P450arom；rat 多囊卵巢