地灵丹汤对单侧输尿管梗阻大鼠模型肾间质纤维化的防治作用_临床医学论文.docVIP

地灵丹汤对单侧输尿管梗阻大鼠模型肾间质纤维化的防治作用_临床医学论文 地灵丹汤对单侧输尿管梗阻大鼠模型肾间质纤维化的防治作用_临床医学论文 作者：宦红娣, 张景红, 郑士荣, 孟巍巍, 张军力 【摘要】 　　目的：研究中药复方地灵丹汤对单侧输尿管梗阻（unilateral ureteral obstruction，UUO）致肾间质纤维化模型大鼠肾间质纤维化的防治作用及其可能机制。　　方法：将60只SD大鼠随机分为假手术组、模型组、依那普利组及地灵丹组。采用UUO法建立肾间质纤维化大鼠模型，观察血尿素氮、血清肌酐和24 h尿蛋白定量及肾组织病理改变，并采用免疫组织化学法检测转化生长因子β1（transforming growth factor）、α平滑肌肌动蛋白（α）、纤维连接蛋白（fibronectin, FN）及层黏连蛋白（laminin, LN）的表达。　　结果：第7天模型组肾间质大量炎症细胞浸润及胶原表达，TGF、FN和LN表达面积百分率较假手术组明显增加（0.05）；地灵丹组间质纤维化面积较模型组减少（0.05）。第14天地灵丹组和依那普利组肾间质TGF、α、FN和LN表达面积百分率较模型组减少（0.05），BUN、SCr和24 h尿蛋白无明显差异。第21天地灵丹组SCr、肾间质纤维化和TGF表达面积百分率较依那普利组和模型组下降（0.05）。　　结论：地灵丹能减轻单侧输尿管梗阻模型大鼠的蛋白尿和肾间质纤维化，抑制TGF、α、FN和LN的表达。在较长时间应用后，地灵丹对模型大鼠的肾功能保护作用优于依那普利。 【关键词】 地灵丹; 肾间质纤维化; 转化生长因子β1; α平滑肌肌动蛋白; 纤维连接蛋白; 层黏连蛋白; 大鼠 　　Objective: To investigate the preventive and therapeutic effects and the possible mechanisms of Dilingdan Decoction (DLDD), a compound Chinese herbal medicine, on rats with renal tubulointerstitial fibrosis induced by unilateral ureteral obstruction (UUO). 　　Methods: Sixty SD rats were randomly divided into shamd kidney were observed. The expressions of transforming growth factorβ1 (TGFβ1), alphasmooth muscle actin (αSMA), fibronectin (FN) and laminin (LN) were detected by immunohistochemical method and coloredmultimedia pathological image analysis system. 　　Results: Massive inflammatory infiltrates and collagen expression in renal interstitial in the untreated group were observed on the 7th day. Compared with the shamd group were markedly increased (0.05), while the percentage of area of interstitial fibrosis was decreased in the DLDDtreated group as compared with the untreated group (0.05). On the 14th day, the percentages of area of TGFβ1, αSMA, FN and LN expressions were declined in two treated groups as compared with the untreated group (0.05), but had no statistical difference in biochemical indicators, including BUN, SCr and 24hour urinary protein. On the 21st day, the level of SCr and the percentage of area of TGFβ1 expression in the