基质金属蛋白酶-3、10在子宫内膜腺癌组织中的表达_临床医学论文.docVIP

基质金属蛋白酶-3、10在子宫内膜腺癌组织中的表达_临床医学论文 基质金属蛋白酶-3、10在子宫内膜腺癌组织中的表达_临床医学论文 作者：冯骏，苟文丽，刘丹，李旭 【摘要】 目的 了解基质金属蛋白酶-3、10(MMP-3、MMP-10)在子宫内膜腺癌组织中的表达情况，及与病变的浸润程度、组织学分级、临床分期等方面的关系，探讨MMP-3、MMP-10在子宫内膜癌的发病、浸润和转移中的作用及意义。方法 采用免疫组织化学方法分别检测42例子宫内膜癌、12例非典型增生、12例正常子宫内膜组织中MMP-3、MMP-10的表达，并进行统计学分析。结果 本实验结果显示，子宫内膜腺癌、子宫内膜不典型增生过长和正常子宫内膜组织中MMP-3阳性表达率分别为73.8%(31/42)、25.0%(3/12)、8.3%(1/12)，MMP-10阳性表达率分别为76.2%(32/42)、33.3%(4/12)、0%(0/12)。MMP-3、MMP-10的表达均呈逐渐下降趋势，每两组的统计学检验显示，MMP-3、MMP-10在子宫内膜腺癌与子宫内膜不典型增生过长、子宫内膜腺癌与正常子宫内膜组织之间，均有统计学差异(0.01)；；MMP-3、MMP-10的表达与组织学分级、肌层浸润程度、临床分期均有关(0.01)；MMP-3、MMP-10在子宫内膜腺癌中的表达有明显相关性(0.01)，联合检测MMP-3、MMP-10可提高子宫内膜腺癌的检出率。结论 MMP-3与MMP-10可作为子宫内膜腺癌的肿瘤标志物之一，且联合检测MMP-3与MMP-10可提高子宫内膜腺癌的检出率。 【关键词】 子宫内膜腺癌；基质金属蛋白酶-3；基质金属蛋白酶-10 ChinaABSTRACT: Objective To explore the expression of matrix metalloproteinase-3 (MMP-3) and matrix metalloproteinase-10 (MMP-10) in normal endometrium, atypical hyperplasia and endometrial adenocarcinoma. We aimed to study the relationship of MMP-3 and MMP-10 expressions with infiltration depth, histologic grade and clinical phase in order to explore the role of MMP-3 and MMP-10 in the occurrence, infiltration and metastasis of endometrial adenocarcinoma. Methods The expressions of MMP-3 and MMP-10 were measured by immunohistochemistry in 12 cases of normal endometrium, 12 cases of atypical hyperplasia and 42 cases of endometrial adenocarcinoma. Results The expression of MMP-3 was 73.8% (31/42), 25.0% (3/12) and 8.3% (1/12) in endometrial adenocarcinoma, atypical hyperplasia and normal endometrium, respectively, whereas the expression of MMP-10 was 76.2% (32/42), 33.3% (4/12) and 0(0/12), respectively. Both MMP-3 and MMP-10 expressions exhibited a pattern of decreased intensity in endometrial carcinoma, atypical hyperplasia and normal endometrium. Furthermore, the statistical analysis showed that the expression of both proteins was significantly greater in endometrial carcinoma than in atypical hyperplasia and normal tissue (0.01). Expressions of MMP-3 and MMP-1