姜黄素抗As2O3诱变作用的实验研究_临床医学论文.docVIP

姜黄素抗As2O3诱变作用的实验研究_临床医学论文 姜黄素抗As2O3诱变作用的实验研究_临床医学论文 【摘要】 目的：研究姜黄素抗三氧化二砷（As2O3）的诱变作用。方法：分别采用小鼠骨髓细胞和人外周血淋巴细胞微核（MN）实验，计数含有MN的嗜多染红细胞和淋巴细胞数，计算MN率。观察As2O3细胞诱变性及不同浓度姜黄素对As2O3诱变性作用的影响。结果：①小鼠骨髓MN试验：与As2O3组比较，As2O3+1.2 mg/mL，2.4 mg/mL，4.8 mg/mL姜黄素组小鼠骨髓细胞MN率显著降低（P＜0.05，P＜0.01）。②人外周血淋巴细胞MN试验：与空白对照组比较，1 μg/mL、2 μg/mL、4 mg/mL As2O3组人外周血淋巴细胞MN率升高（P＜0.01）；与1 μg/mL（2 μg/mL、4 μg/mL）As2O3组比较，5 μg/mL，10 μg/mL，20 μg/mL姜黄素+1 μg/mL（2 μg/mL，4 μg/mL）As2O3组MN率降低（P＜0.05，P＜0.01）。结论: As2O3具有剂量相关的致突变作用，姜黄素具有明确的抗As2O3诱变性作用。 【关键词】 姜黄素；三氧化二砷；诱变性 Abstract Objective：To observe the inhibitive effect of curcumine against mutagenicity of As2O3 . Methods：①Micronucleus（MN） frequency test in bone marrow of mice: 60 mice were randomly divided into blank control group，As2O3 group and 1.2 μg/mL，2.4 μg/mL，4.8 μg/mL curcumine+As2O3 group（n=12）.Blank control group and As2O3 group were administered salad oil through intragastrical perfusion for 7 days.Then blank control group was injected intraperitoneally with 0.5 mL saline，other three groups were injected with 0.5 mL As2O3 solution（20 μg/mL）.24 h later，mice were sacrificed and MN frequency test in bone marrow of mice was made.②MN frequency test in peripheral blood lymphocyte of human：human blood samples were divided into 13 groups：blank control group，1 μg/mL，2 μg/mL，4 μg/mL As2O3 group，5 μg/mL，10 μg/mL，20 μg/mL curcumine+1 μg/mL（2 μg/mL，4 μg/mL）As2O3 group.Then MN frequency test in peripheral blood lymphocyte of human was made to study mutagenicity of As2O3.Results：①Compared with As2O3 group，MN frequency in 1.2 mg/mL，2.4 mg/mL，4.8 mg/mL curcumine+As2O3 group were significantly decreased（P＜0.05，P＜0.01）.②Compared with 1 μg/mL （2 μg/mL，4 μg/mL） As2O3 group，MN frequency in 5 μg/mL，10 μg/mL，20 μg/mL curcumine+1 μg/mL （2 μg/mL，4 μg/mL） As2O3 group were significantly decreased（P＜0.05，P＜0.01）.Conclusion：As2O3 has dosage-correlative mutagenic effect，and curcumine has a remarkable antimutagenic effect against As2O3. Key words curcumine，As2O3，mutagenicity 姜黄素（curcumine