姜黄素脂质体的制备及其体外抗脂质过氧化的研究_临床医学论文.docVIP

姜黄素脂质体的制备及其体外抗脂质过氧化的研究_临床医学论文 姜黄素脂质体的制备及其体外抗脂质过氧化的研究_临床医学论文 【摘要】 目的 制备姜黄素脂质体,并考察其在体外对大鼠组织脂质过氧化的影响。方法 采用薄膜分散挤出法制备姜黄素脂质体,微柱离心法测定姜黄素脂质体的包封率(encapsulation efficiency,EE),并以包封率为指标通过正交试验对处方进行筛选。采用硫代巴比妥酸(TBA)法检测大鼠组织脂质过氧化产物丙二醛(MDA)的生成量,确定姜黄素脂质体对大鼠组织脂质过氧化的影响。结果 按最佳处方制备的姜黄素脂质体包封率为(89.32±1.58)%.在体外,姜黄素脂质体对大鼠心、肝、肾、脑脂质过氧化的抑制率分别为70.02%、59.48%、59.10%、85.21%.且在试验范围内随着姜黄素脂质体加入量的增加,抑制率递增。结论 薄膜分散挤出法制备姜黄素脂质体简单易行,在体外姜黄素脂质体对大鼠组织脂质过氧化有明显的抑制作用。 【关键词】 姜黄素;脂质体;包封率;正交设计试验;脂质过氧化 　　【Abstract】 Objective To prepare curcumin liposomes and study its impact on the lipid peroxidation in rat tissues in vitro.Methods Curcumin liposomes were prepared by film dispersion and extrusion technique.Then encapsulation efficiency(EE)of curcumin liposomes was determined by minicolumn centrifugation method.Orthogonal experimental design was used to optimize the formulation.To evaluate the impact of curcumin liposomes on the lipid peroxidation in rat tissues,thiobarbituric acid(TBA)method was adopted to detect the amounts of malondialdehyde(MDA),a product of lipid peroxidation reaction.Results The average EE of curcumin liposomes prepared according to the optimized formulation was(89.32±1.58)%.The inhibition rate of curcumin liposomes on rat hearts,livers,kidneys and brains lipid peroxidation was 70.02%,59.48%,59.10%,85.21%,respectively in vitro.In addition,as the amount of curcumin liposomes increased,the inhibition rate increased accordingly.Conclusion Using film dispersion technique for preparation of curcumin liposomes is simple and feasible.Curcumin liposomes can significantly inhibit lipid peroxidation in rat tissues in vitro. 　　【Key words】 curcumin;liposomes;encapsulation efficiency;orthogonal experimental design;lipid peroxidation 　　医学研究表明,许多疾病与自由基和脂质过氧化密切相关,如癌症、自身免疫性疾病、糖尿病和衰老等[1]。为了抑制自由基的产生和脂质过氧化,尤其是在人体的保护系统功能减弱时,常需要提供外源性抗氧化剂,用于阻断自由基对细胞的氧化性损伤,保护人体细胞。 　　姜黄素(curcumin)是姜科姜黄属(Curcuma longa L.)植物姜黄、莪术、郁金根茎中分离出的活性物质之一,具有广泛的药理作用如抗炎、抗氧化、清除自由基及抗肿瘤等[2]。但姜黄素不溶于水,在人体内生物利用度低,且在中性和碱性的环境中易降解,给临床应用带来很大的局限性[3]。脂质体作为药物载体,