兔骨骼肌ryanodine受体%2f钙释放通道聚集的AFM的研究.pdfVIP

2001增刊 压电与声光 501 文章编号：1004-2474(2001)S0·0501-03 兔骨骼肌ryanodine受体／钙释放 通道聚集的AFM研究 胡晓芳1，魏青青2，陈克樱2，朱培闳2，胡钧1，李明乾3 (1上海交通大学Bio-X中心蚋米生物学实验室，上海200030；2中国科学院上海生理所，上海20003 3中国科学院上海原子棱所蚋米切割和蚋米操纵实验室，上海200800) 个亚基的分子量的为550-Kda，是目前已知的最大的离子通过．RyR在内质网膜上常常形成二堆晶格排列．近期的理 论研究表明爱体膜蛋白的这种聚集方式为细胞信号传导提供了一种新的方式．实验发现，适当比例的RyR和磷脂(含 堆聚集进行了研究． 关键词；Hyanodine受体裁(RyR)；=堆聚集；原干力显微镜(AFM) 中图分类号tTH742 文献标识码；A Dimensional Researchof Two Aggregation Ryanodine ReleaseChannelwithAFM (LaboratoiTofNanobiology,Bio-XLife 200030，China) ScienceResearchCenter,SharIghaiJiaoTongUnivcrsi吼Shan曲ai Abstract：The release the membrane locatedinthe ryanodinereceptor／calciumchannel，aSlargestintegral protein，is ofintracellular and an roleincalciumreleasein and membrane Ca2+-storingorganellesplaysimportant brain，heart，skeletal smooth and intheirextensivestudiesofthenative reticulummembrane muscle．Franzini-Armstrongcolleagues sarcoplasmic forma inthe theoreticaland evidence showed semicrytalline ofthemembrane．Recent RyRs array plane analysisexperiment hasindicatedthatsuch inthemembranecould increasetheir toactivationof clustering dramatically sensitivity ligandby for incells switchedonoroffoftheclustered isanovelmechanism simultaneously RyRs．This regulationreceptorsignalling Inour fotmdanewmethodto thetwodimensional withAFM．Whenthemixtureof research，we study aggr