乳腺癌组织中APC与Bikunin基因异常甲基化.pdfVIP

空生塑笾匡堂苤盍!Q盟生鱼旦筮垒!鲞缝型垦塾垫』堕!丛鲤：也塑呈螋!：!丛堡!：§业P堡翼!堕 。论著． 陈扬霖解云涛文贤子邓大君 【摘要】 目的探讨APC和Bikunin基因甲基化状态与乳腺癌临床病理特征之间的相关性。 方法采用甲基化特异性聚合酶链反应法检测152例乳腺癌组织中APC和Bikunin基因的甲基化状 态。结果乳腺癌组织中APC基因启动子区CpG岛甲基化频率为40．8％，其甲基化状态与肿瘤大 小肴相关(x2=4．041；|P=0．044)，但是与患者年龄、生存期、肿瘤的病理类型、组织学分级、临床分 期、淋巴结转移以及雌激素或孕激素受体等状态无相关性；Bikunin基因启动子区CpG岛甲基化频率 为24．6％，与肿瘤的临床病理学特征无相关性。结论APC和Bikunin基因启动子区CpG岛异常甲 基化是乳腺癌组织中的频发事件，与乳腺癌的预后无相关性。 【关键词】乳腺肿瘤； 甲基化；CpG岛；基因，APC Aberrant of andBikunin islandsin breastcarcinomas APC CpG sporadic methylation School and CHEN UniversityofOncology Yang—h。XIEYun-tao，WENXian-zi，DENGDa-jugPeking Cancer Institute Being for Research，莉喟100036，China corn authors：五腰Yun—tao。WENXian-zi，Email：wenxianzi@hotmaiL Corresponding oftheAPCand To between status 【Abstract】0bjecfiveinvestigaterelationshipmethylafion The Bikuninislandsand characteristicsofbreastcarcinomas．Methods CpG elinicopathologi【cal methylation statusofAPCandBikuninislandsin152 breastcarcinomawere CpG sporadic samplesanalyzedby PCR．Results40．8％ofbreastcarcinomasexaminedshowed for methylationspecific methylatedsignals theAPC．The ofAPCwas correlatedtotlletumor methylationfrequency significantly size(∥=4．041；P= notto of node 0．044)，butpatients’age，pathologictypetumor，clinicalstage，histologicalgrade，lymph statusof or addition．24．6％ofcarcinoma met