三种不同生物学特性细胞在软琼脂中的生长表型分析.pdfVIP

国肺癌杂志 2008 年 2 月第 11 卷第 1 期 Chin J Lung Cancer, February 2008, Vo11, No.1 51 窑基础研究窑 种不同生物学特性细胞 在软琼脂中的生长表型分析 刘芳莉 于旸 傅松滨 摘要铱 背景与目的 软琼脂 soft agar冤实验一般用来检测细胞的集落形成能力袁但很早就有人根据形 态学变化用它来检测低等生物的生物学特性遥 本文探讨利用软琼脂集落形成实验这种方法进行 乳动物癌细 胞表型初步分析的可行性遥方法 将三种不同生物学特性的细胞 小鼠成纤维细胞NIH3T3 袁高转移人肺腺癌细 胞系Anip973袁高浸润人肺腺癌细胞系L18冤悬液接种到软琼脂 袁分别在 1 天尧5 天尧14 天后观察细胞形态特 征遥 结果 第五天开始细胞集落形态出现差异袁14 天时差异更显著遥 NIH3T3 细胞克隆为椭圆形袁 等大小袁边 界清楚曰Anip973 克隆周围有大量游走细胞袁表现出其转移特性曰L18 细胞克隆分支多并向周围延伸扩展袁与其 浸润特性相符遥结论 软琼脂分析实验是一种良好的根据表型来观察及鉴定 乳动物细胞生物学特性的方法遥 关键词铱 软琼脂 细胞学 细胞培养 中图分类号铱 Q-331 DOI: 10.3779/j.issn.109-3419.2008.01.013 The morphous analyses of three different characteristics cells growing in soft agar LIU Fangli, Y U Y ang, FU Songbin.Laboratory of Medical Ge netics, Harbin Medical Univ ers ity,Harbin , Heilongj iang 150081,China Corresponding author: FU Songbin, E-mail: fusb@ems . Abstract铱 Background and obj ective Soft agar assay was usually used in colony formation observation of cells, while it was also be empolyed in detecting bionomics of inferior creature. In this article the feasibility of soft agar assay in detecting mammalian cell phenotype is investigated. Methods Three different characteristic cells( L cell NIH3T3 曰 higher metastatic human lung adenocarcinoma cell line Anip973 曰 higher invasive potential human lung adenocarcinoma cell line L18) were seed into soft agar. The morphology of cells was obserned in the first day 袁 the fifth day and the fourteenth day 袁respectively. Results In the f