吡罗昔康固体脂质纳米粒制备及其经皮吸收及研究.pdfVIP

吡罗昔康固体脂质纳米粒的制备及其经皮吸收的研究 1,2 1 1 1 2 1 1 魏巍 、单颖慧 、余振伟 、彭丽华 、于莲 、梁文权 、高建青 * （1浙江大学药学院；2佳 木斯大学药学院）*:通讯作者，gaojianqing@zju.edu.cn 摘要 目的：制备吡罗昔康固体脂质纳米粒并考察其体外经皮特性。方法：采用乳化超 声法制备固体脂质纳米粒，正交实验设计并优化处方，采用Franz透皮实验仪, 以 大鼠背部皮肤为模型, 高相液相测定药物透皮浓度，荧光显微镜及激光共聚焦显 微镜观察其透皮机制。结果：固体脂质纳米粒较优处方包含，吡罗昔康1%、单 硬脂酸甘油酯20% 、卵磷脂15%、吐温1%，纳米粒外观形态圆整，呈类球形实体 粒子，吡罗昔康固体脂质纳米粒渗透速率可达1.02±0.041 μg/(cm2 ·h) ，经皮途 径主要为毛囊、汗腺等皮肤附属器。结论：吡罗昔康固体脂质纳米粒具有一定的 透皮能力，有望成为吡罗昔康经皮给药新剂型。 关键词：吡罗昔康; 固体脂质纳米粒; 经皮吸收 Study on preparation and percutaneous characeristics in vitro of piroxicam solid lipid nanoparticle 1,2 1 1 1 2 1 1 Wei Wei 、Shan Yinghui 、Yu Zhenwei 、Peng Lihua 、Yu Lian 、Liang Wenquan 、Gao Jianqing * （1 College of Pharmaceutical Sciences, Zhejiang University；2 College of Pharmacy, Jiamusi University ）*:Corresponding author，gaojianqing@zju.edu.cn Abstract Objective: To prepare solid lipid nanoparticles of piroxicam(Pir-SLN) and investigate its transdermal characteristics in vitro. Methods: Piroxicam loaded SLN were prepared with the Emulsification ultrasonic method by orthogonal experiment design. The transdermal characteristics were evaluated by Franz diffusion cell method, Rats skin was used as permeation barrier and the drug concentration was measured by HPLC, the characterization of skin after skin permeation were investigated by fluorescence microscope, laser confocal scanning microscopy. Results: The optimum formulation consisted of 1% Piroxicam, 20% Glycerin monostearate, 15%Lecithin, 1%Tween, the obtained SLN were spherical, the permeation rate of Piroxicam was 1.02±0.041 μg/(cm2 ·h), SLN can penetrate the skin barrier through the skin appendages pathway. Conclus