与抗逆相关的毛竹WRKY转录因子基因对外源激素及UV-B胁迫的响应.pdfVIP

第六届中 竹业学术大会 竹类生物学基础 与抗逆相关的毛竹WRKY 转录因子基因对外源激素及UV-B 胁迫的 响应 崔晓伟，齐飞艳，陈媛文，高健* ( 际竹藤网络中心， 家林业局竹藤科学与技术重点实验室，北京 100102) 摘 要：WRKY 转录因子家族的成员广泛参与植物抗逆反应和多种生理代谢过程。为了研究WRKY 蛋白 在毛竹中的作用，本研究克隆了一个PheWRK Y10 基因，GenBank 登录号GU944762 。序列分析表明，该 基因全长 1080bp，包括一个579bp 开放阅读框，推测编码一个由193 个氨基酸组成的蛋白质。序列分析 结果表明，Phe WRK Y10 基因编码蛋白的保守元件与禾本科植物甘蔗、水稻、小麦、黑麦草等有很高的一 致性，都在86% 以上。构建PheWRK Y10 基因植物表达载体，质粒酶切鉴定表明该表达载体构建成功。实 时荧光定量PCR 结果显示，Phe WRK Y10 在毛竹中的表达受水杨酸(salicylic acid, SA) 、茉莉酸(j asmonic acid, JA)和紫外线辐射的诱导，由于SA 和JA 在介导植物抗病反应过程中存在“对话”关系，表明PheWRK Y10 对生物与非生物胁迫都有应答反应。 关键词：毛竹；WRKY 转录因子；表达载体；实时荧光定量PCR The Responsive of WRKY Transcriptional Factor Gene Related to Stress Tolerance in Moso Bamboo (Phy llostachys edulis) to Exogenous Hormone and Enhanced UV-B Radiation * Cui Xiao-Wei Qi Fei-Yan Chen Yuan-Wen Gao Jian ( International Centre for Bamboo and Rattan, Key Laboratory of Bamboo and Rattan Science and Technology of the State Forestry Administration Beij ing 100102) Abstract: Members of plant WRKY transcription factor families are widlely implicated in defense responses and various other physiological processes. To study the function of WRKY transcription factors in moso bamboo (Phy llostachys edulis), a bamboo Phe WRK Y10 gene was cloned. Sequence analysis indicated that the full-length sequence of the bamboo Phe WRK Y10 gene was 1080 bp, and contained an open reading frame (ORF) of 579 bp encoding a 193 amino acids protein.The results of sequence analysis showed that the conserved sequences of the protein encoded by Phe WRK Y10 were highly homologous to the proteins from Saccharum sinense, Oryza sativa L., Triticum aestivum Linn. and Lolium p erenne L. with