FUT1基因多态性及其和产仔性状的关联性研究.pdfVIP

HEREDITAS (Beijing) 2007 1 , 29(1): 52―56 ISSN 0253-9772 研究报告 DOI: 10.1360/yc-007-0052 FUT1 基因多态性及其与产仔性状的关联性研究 1 2 2 张引红, 周忠孝 , 曹果清 1. , 030001; 2. , 030801 : 采用PCR-RFLP 技术6 个中外品种共245 头猪的FUT1 基因进行了研究, 结果表明, Hin 6 Ⅰ位点上, 大白 猪、长白猪和杜洛克猪3 个外来猪种均存在多态, 且以敏感型(GG 型和AG 型)居多; 山西黑猪、太原花猪和马 身猪3 个本地猪种的所有检测样品都表现为GG 型。用方差分析方法分析FUT1 基因型、品种和胎次与产仔性 状之间的关系, 基因型和品种对猪的总产仔数的影响显著, 胎次对总产仔数影响不显著。而基因型、品种和胎 次对产活仔数影响均不显著。 : 猪; FUT1 基因; PCR-RFLP; 产仔性状 FUT1 gene polymorphism and its association with litter size in pigs 1 2 2 ZHANG Yin-Hong , ZHOU Zhong-Xiao , CAO Guo-Qing 1. Department of Laboratory Animal Science , Shanxi Medical University, Taiyuan 030001 China; 2. College of Animal Science and Technology, Shanxi Agriculture University, Taigu 030801 China Abstract: The genetic variations of FUT1 gene at nucleotide position 307 of its open reading frame were investigated by PCR-RFLP in a total of 245 pigs from 3 exotic pig breeds and 3 Chinese native pig breeds. Results showed that the genetic polymorphisms of the FUT1 locus were only detected in the 3 exotic pig breeds. There were 3 different genotypes and the majority were the susceptible forms including GG and AG . All Chinese native pig breeds only presented the susceptible genotype GG. Univariate analysis of variance components indicated that FUT 1 gene and the type of breeds significantly affected total number born, whereas parities did not. None of these factors significantly affected the number of liveborns. Keywords: pig; FUT1 gene ; polymorphisms ; litter trait , , [3,4] 11.5%~ , Vögeli [5] 29.5%[1