薯蓣皂苷元的制备与其抗甲亢活性研究.pdfVIP

薯蓣皂苷元的制备及其抗甲亢活性研究 秦龙，胡雪剑，任建功，焦海胜，李平*(兰州大学第二医院，兰州 730030) 1 摘要：目的 考查薯蓣皂苷元对甲亢大鼠的活性。方法 穿山龙饮片用含 1.5 mol·L 硫酸的 75%乙醇水溶液在沸水浴中 回流提取 4.5 h ，再经柱层析法分离、精制纯化得薯蓣皂苷元提取物，并进行结构表征。采用优甲乐灌胃法制作大鼠甲亢 模型，并将大鼠随机分成正常对照组、甲亢模型组、甲巯咪唑治疗组、薯蓣皂苷元治疗组(低、中、高剂量) ，分别于给药 21 d 后测定大鼠血清中 T3 、T4 及 TSH 值。结果 薯蓣皂苷元提取制备工艺正确适当，所得薯蓣皂苷元提取物经氢谱、 碳谱、质谱和高效液相色谱表征确定为薯蓣皂苷元。与甲亢模型组比较，薯蓣皂苷元治疗组(低、中、高)血清中 T 、T 3 4 和 TSH 值均有不同程度改善。结论 薯蓣皂苷元制备工艺合理可行，得率较高。薯蓣皂苷元可使甲亢大鼠的各项甲状腺 功能血清值恢复正常，还可增加其体质量，功效与甲巯咪唑相似，值得进一步研究。 关键词：穿山龙；薯蓣皂苷元；模型；甲亢 中图分类号：R285.5 ；R284.2 文献标志码：A 文章编号：1007-7693(2015)06-0692-04 DOI: 10.13748/ki.issn1007-7693.2015.06.012 Preparation and Study on Anti-hyperthyroidism Activities of Diosgenin * QIN Long, HU Xuejian, REN Jiangong, JIAO Haisheng, LI Ping (Lanzhou University Second Hospital, Lanzhou, 730030, China) ABSTRACT: OBJECTIVE To evaluate the efectiveness of diosgenin on hyperthyroid rats. METHODS Dioscoreae Nipponicae Rhizoma was refluxed for 4.5 h in 75% ethanol aqueous solution which containing sulfuric acid 1.5 mol·L1. The crude product was purified by column chromatography on silica gel, and the structure of diosgenin was characterized. The model of hyperthyroid rats was prepared by intragastric administration of euthyrox, the rats were randomized into six groups: The normal rats group, the model group, the methimazole group, the diosgenin groups(low, medium and high dose). T , T and TSH 3 4 in rats serum were examined separately after 21 days. RESULTS The diosgenin structures were