解淀粉芽孢杆菌Q-426中天然环二肽的发现及其性质的初步的研究.pdfVIP

解淀粉芽孢杆菌Q-426 中天然环二肽 的发现及其性质的初步研究 王建华1,2权春善3 **王雪1,2赵朋超1,2范圣第3 （1 中国科学院大连化学物理研究所 大连 116023 ； 2 中国科学院研究生院 北京 100049 ； 3 大连民族学院生命科学学院 大连 116600 ） 摘要：本实验室从堆肥中分离得到一株具有抗真菌活性的菌株解淀粉芽孢杆菌 Q-426 ，通过对其发酵液的 乙酸乙酯萃取物中的化学成分进行研究，分离得到5 种不同的环二肽类化合物，利用气相色谱-质谱(GC-MS) 鉴定其分别为：Cyclic (Pro-Phe) ，Cyclic (Pro-Tyr)，Cyclic (Pro-Leu) ，Cyclic (Pro-Val)，Cyclic (Ala-Val)。抑 菌圈实验表明这些环二肽本身对一些真菌的生长具有抑制作用。值得关注的是，还发现这类化合物可以作 为群体感应信号分子对解淀粉芽孢杆菌Q-426 的抑菌活性及生物膜的形成有一定的抑制作用。 关键词：解淀粉芽孢杆菌Q-426 ，环二肽，气相色谱-质谱，群体感应，抑菌圈实验 中图分类号: TQ02 UDC: 66.0 Discovery and characterization of the natural cyclic dipeptides in Bacillus amyloliquefaciens Q-426 Wang Jianhua1,2 Quan Chunshan3** Wang Xue 1,2 Zhao Pengchao 1,2 Fan Shengdi 3 ( 1 Dalian Institute of Chemical Physics, Chinese Academy of Sciences Dalian 116023 ， china; 2 Graduate School of Chinese Academy of Sciences Beijing 100049 ， china; 3 Department of Life Science , Dalian Nationalities University Dalian 116600 ，china) Abstract: The strain of Bacillus amyloliquefaciens Q-426 with great antifungal activity was isolated from the compost in Dalian. The chemical composition of the ethyl acetate extract of B. amyloliquefaciens Q-426 was studied. 5 different cyclic dipeptides were got, and GC-MS analysis shows that they are Cyclic (Pro-Phe), Cyclic (Pro-Tyr), Cyclic (Pro-Leu), Cyclic (Pro-Val), Cyclic (Ala-Val). Inhibition zone experiment shows that they have antifungal activity, and moreover these compounds can serve as quorum sensing signal molecules of B. amyloliquefaciens Q-426, and regulate the antifungal activity and the biofilm formation of Q-426. Key words: Bacillus amyloliquefaciens Q-426, cyclic dipeptides, GC-MS, quorum sensing, inhibition zone