四川粗壮女贞苦丁茶中黄酮的提取_分离和初步鉴定_叶善蓉.pdfVIP

第 22 卷 　第 2 期 四川农业大学学报 Vol . 22 No . 2 　　　　　　　　 　 　　　　　　　　 　 2004 年 06 月 Journal of Sichuan Agricultural University J un . 2004 Extraction , Separation and Primary Identif ication of Flavonoids f rom L igust r u m r obust u m in Sichuan YE S h a nr on g , TAN G Qi a n , DU Xi a o (College of Forestry and Horticulture , Sichuan Agricultural University , Yaan 6250 14 , Sichuan , China) Abstract : By using L ig us t r u m robus t u m ( R x ob. ) BL . in Sichuan as materials and t he experiment of L 9 (34 ) by et hanol as ext racting way , t he optimum conditions of ext racting flavonoids from L ig us t r u m robus t u m are obt ained . When t he condition is 80 % of density of et hanol , 80 ℃of ext racting tempera t ure , 10 ∶1 et hanol volume and sample weight , 4 h of ext racting time , t he ext racting effect is better , t he ext racting rate can reach 84 32 % and t he tot al content of flavonoids is 47 6803 mg/ g . Then t he ext raction are sep arated and p urified to gain six kinds of compounds. Primary identification of t he st ruct ure by t hinlayer chromatograp hic p rocedure of silica GF254 and t he color reactions show t hat com pound A are isoflavones , compound B , D , F are all flavones (or flavonols) , compound C are flavanones (or flavanonol) , compound E are ant hocyanins. Key words : L ig us t r u m robus t u m ; Kudingcha ; flavonoids ; ext raction ; t hinlayer chromatograp hic 四川粗壮女贞苦丁茶中黄酮的提取 、分离与初步鉴定 叶善蓉 , 唐 　茜 , 杜 　晓