GnRH在广西摩拉杂交水牛乳腺的免疫组化定位及其受体基因表达的研究.pdfVIP

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  • 2018-03-24 发布于安徽
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GnRH在广西摩拉杂交水牛乳腺的免疫组化定位及其受体基因表达的研究.pdf

PCRtodetectedgonadotropicreleasinghormone GnRH andgonadotropicreleasinghormone receptor GnRH-R inthemammaryglandofGuangxihybridMurrahBuffalo.Theresultshow that13piecestissuewerepositivein15piecesnormalmammaryglandtissue,thepositivesubstancewere mainlydistributedinperiplastandmembraneofacinouscellanditwasnegativeinnucelus;Meanwhile, theGnRH-Rgenefragmentwasamplifiedwith1008bpinsize.Thestudypavedthewayforelucidating themechanismofmammaryglandfunctionregulationbyGnRH. Keywords:gonadotropicreleasinghormone;gonadotropicreleasinghormonereceptor;mammarygland; Immuno-histochemical g模板,进行RT.

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