斑马鱼CDKAL1基因生物信息学分析.pdfVIP

安徽农业科学,Journal of Anhui Agri . Sci .2008 ,36(24) :10356 - 10358 责任编辑 王淼 责任校对 况玲玲 斑马鱼 CDKAL 1 基因的生物信息学分析 焦传珍 ( 德州学院生物系, 山东德州253023) 摘要 [ 目的] 深入 了解斑马鱼 CDKAL1 基 因的结构特点。[ 方法] 运用生物信 息学方法对斑马 鱼 CDKAL1 基 因的基 因序列、编码蛋 白的 理化性质、结构及功能进行分析 。[ 结果] 分析结果显示, 此基 因一部分存在于细胞质, 并存在 一个跨膜结构域, 没有信号肽。该 基 因编 码蛋 白质的变异不大; 含有3 个功能结构域, 均为Erk D- domain , 分别位于L189 、L384 、V315 ; 在Y292 位置有一磷酸化位 点; 有UPF0004 超 家 族、Radical SAM 超 家族和 MiaB 结构域3 个保 守结构域; 在脑 、肌 肉、肾脏 中表达。推测该基 因表达的蛋 白质可能是酶、异构酶或者 中间 代谢产物, 与胰 岛素受体 的作用有关。[ 结论] 该研 究为今后进一步研 究人 类Ⅱ型糖尿病提供 了科学依据。 关键词 斑马鱼; CDKAL1 基 因; 生物信 息学 中图分类号 S9 17 文献标识码 A 文章编号 0517 - 6611(2008) 24 - 10356 - 03 Bioinfor matics Analysis of CDKAL1 Gene from Dani o r eri o JI AO Chuan-zhen ( Department of Biology ,Dezhou University ,Dezhou ,Shandong 253023) Abstract [ Obj ective] The ai mwas to understand the structural characteristics of CDKAL1 gene from Danio reri o deeply .[ Method] The sequence of CD- KAL1 gene from D .reri o and the physical and chemical properties ,structure and function of its encoding protein were analyzed using the bioinfor matics method .[ Result] The analysis result showed that the gene existed in the cytoplasmpartially ,had atrans- membrane domai n and without signal peptide .The protein encoded by the gene had no significantly variation ;it had 3 functional domai ns which was all Erk D- domain and located at L189 ,L384 and V3 15 , resp . ;there was a phosphorylation site at the location of Y292 ;the protein had 3 conserved domai ns of UPF0004 superfamily ,Radical SAMsuperfamily and MiaB domai n ;it expressed in brain ,muscle and kidney .It was conj ectured that the protein expressed by the gene could be enzyme ,isomerase or inter medi- ate metabolite and it had relation with the function of insulin receptor .[ Conclusion] This research provided the scientific basis for the further study of hu- man T