棉花4个脂肪酸合成相关基因的克隆与表达特征分析.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2010, 36(12): 2084−2090 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2010.02084 棉花4 个脂肪酸合成相关基因的克隆和表达特征分析 董 佳 魏利斌 胡 艳 张天真 郭旺珍* / , 210095 : , 4 , GhKASII GhKASIIIGhFAD GhGPAT, GhKASIIIGhFAD GhGPAT cDNA GhKASII 5′RACE , 4 , GhKASIIGhKASIII 25 DPA , GhGPAT 0 DPA 15 DPA , GhFAD 0 DPA , 15 DPA , 20 DPA , 4 , ABA, : ; ; ; ; Molecular Cloning and Expression Analysis of Four Novel Fatty Acid Synthesis Related Genes in Gossypium hirsutum L. * DONG Jia, WEI Li-Bin, HU Yan, ZHANG Tian-Zhen, and GUO Wang-Zhen National Key Laboratory of Crop Genetics Germplasm Enhancement / Nanjing Agricultural University, Nanjing 210095, China Abstract: Metabolism related to fatty acid synthesis plays an important role both in regulating oil biosynthesis and in plant abiotic stress tolerance. The spatial and temporal expression level of genes related to fatty acid synthesis influences the oil content and enzymes activity for fatty acid synthesis. In this study, four novel genes related to fatty acid synthesis, designated GhKASII, GhKASIII, GhFAD, and GhGPAT, were first isolated from upland cotton, respectively. The cDNA full-length of GhKASIII, GhFAD and GhGPAT were obtained by combining homologic cloning and in sillico cloning, while GhKASII was obtained by screening cDNA library and 5′RACE technique. The expression pattern analysis of different tissues and organs revealed that the transcripts of these genes were widely distributed in all the tested tissues and organs. GhK