脑络安胶囊对大鼠脑缺血再灌注损伤保护作用的实验的研究.pdfVIP

脑络安胶囊对大鼠脑缺血再灌注损伤保护作用的实验研究 张宇燕，万海同，陈伟燕，杜月光，杨洁红 （浙江中医药大学，杭州 310053) 摘要：目的观察脑络安胶囊对大鼠脑缺血再灌注损伤的保护作用。过程与方法 选用SD 大鼠，随机分为假手术 组，生理盐水组，脑络安胶囊不同剂量组，尼莫地平组，每组 10 只。采用大脑中动脉局灶性栓塞（MCAO ）模 型，观察对大鼠神经功能、脑梗死体积、脑含水量、脑组织SOD 活力，MDA 含量，及神经元尼氏小体数等的影 响。结果 脑络安胶囊可以显著地减轻MCAO 模型大鼠的神经功能缺损症状，促进神经功能缺损症状的恢复；显 著地缩小大鼠 MCAO 后脑组织梗死体积，降低脑梗死体积百分率；显著地降低脑缺血模型大鼠的脑指数和脑含 水量；显著地提高缺血再灌注后大鼠脑组织中SOD 活性、明显降低MDA 含量，减轻自由基对脑的损伤；明显改 善脑缺血及再灌注对脑组织的病理损害，抑制尼氏小体数量减少或消失。结论 脑络安胶囊对大鼠缺血性脑损伤 具有明显的保护作用。 关键词：脑络安胶囊；缺血性中风；脑缺血再灌注；保护作用 Study of Protective Effects of Nao Luo An Capsule on Ischemic Brain Injury ZHANG Yuyan, WAN Haitong, CHEN Weiyan, DU Yueguang, YANG Jiehong (Zhejiang Chinese Medical University, Hangzhou 310053, China) Abstract: Objective ：To observe the protective effects of Nao Luo An Capsule on cerebral ischemia and reperfusion injury. Process and Methods: The SD rats were randomly divided into 6 groups: sham-operated group, model group,nimodipine group, and the group of low,medium and high dose of Nao Luo An Capsule. Using models of focal middle cerebral artery embolization (MCAO) model, observe targets including symptoms of neurological deficit score,infarct volume,brain index and brain water content,activity of SOD, content of MDA, and quantity of nissl body. Results:Nao Luo An Capsule could significantly reduce the symptoms of neurological deficits,promote the recovery symptoms of neurological deficits;narrow infarct volume of brain tissue obviously,reduce the percentage of infarct volume; reduce brain index and brain water; raise activity of SOD, reduce content of MDA; inhibit reducing or disappearing of nissl body. Conclusions: There was significant protective effects of Nao Luo An Capsule on ischemic brain injury. Keywords: Nao Luo An C