胆囊良恶性病变组织中蛋白基因产物9.5的表达及临床意义.docVIP

胆囊良恶性病变组织中蛋白基因产物9.5的表达及临床意义 作者：杨晓静 作者单位：中南大学湘雅医学院 病理学系，湖南 长沙 410078 【摘要】 目的 研究胆囊腺癌、癌旁组织、慢性胆囊炎组织中蛋白基因产物9.5（PGP9.5）表达及其临床病理意义。方法 108例胆囊腺癌、46例癌旁组织和35例慢性胆囊炎切除标本常规石蜡包埋切片，PGP9.5染色方法为EnVisionTM免疫组化法。结果 胆囊腺癌PGP9.5表达阳性率（45.4%）和评分（1.94±1.84）明显高于癌旁组织（17.4%，X2=10.83，P0.01；0.86±1.41，t=3.60，P0.01）和慢性胆囊炎组织（11.4%，X2=13.06，P0.01；0.58±1.29，t=4.08，P0.01）；PGP9.5阳性良性病变胆囊上皮均呈中至重度不典型增生。患者年龄≤45岁、腺瘤癌变或高分化腺癌、肿块最大径2 cm、无淋巴结转移和未侵犯周围组织病例PGP9.5表达阳性率及其评分明显低于年龄45岁、低分化腺癌、肿块最大径≥2 cm、淋巴结转移和侵犯周围组织病例（P0.05或P0.01）。结论 PGP9.5表达可能是反映胆囊腺癌发生、进展和预后的重要生物学标志物，检测胆囊良性病例PGP9.5表达水平对于预防和早期发现胆囊癌可能有重要临床意义。 【关键词】 胆囊肿瘤 慢性胆囊炎 蛋白基因产物9.5 免疫组织化学 Expression of protein gene product 9.5(PGP9.5) and clinical significances in the benign and malignant lesions of gallbladder YANG Xiaojing*， YANG Zhulin， Huang Jiangsheng， et al. * Dept. of Pathology， Xiangya Medical College of Central South University， Changsha 410078 Abstract Objective To study the expression of PGP9.5 and its clinicopathological significances in tissues of gallbladder adenocarcinoma， pericancerous tissues and chronic cholecystitis. Methods Immunohistochemical method was used for the detection of PGP9.5 expressive levels in routinely paraffin-embedded sections from the specimens of gallbladder adenocarcinoma (n=108)， pericancerous tissues (n=46) and chronic cholecystitis (n=35). Results The positive rates and scores of PGP9.5 in gallbladder adenocacrcinoma (45.4%， 1.94±1.84) were significantly higher than those in pericancerous tissues (17.4%， X2=10.83， P0.01； 0.86±1.41， t=3.60， P0.01) and chronic cholecysitis (11.4%， X2=13.06， P0.01； 0.58±1.29， t=4.08， P0.01). The positive rates and scores of PGP9.5 were significantly lower in the cases of aged ≤45 years， adenomatous canceration or high-differentiatied adenocarcinoma， maximal diameter of mass 2 cm， no-metastasis of lymphnode， and no-invasion of regional tissues than those in the ones of aged 45 years， low-differentiated adenocarcinoma， maximal diameter of mas