胆道系统癌与血浆k_ras基因突变检测.pdfVIP

# 42 # Acta U niversitatis Medicinalis Secondae Shanghai Vol. 20 Suppl. 2000 : 0258- 5898( 2000) Suppl- 0042- 02 k- ras 1 2 2 ( 1. , 200011; 2. ) : 目的 k - ras 方法 DNA, ( MASA) k- ras codon12 结果 5 k- ras ( 1.4%) , 6 3 2 ( 33. 3%) 1 ( 33. 3%) k- ras k- ras CA 19- 9CEA 1 结论 DNA, k- ras : ras : R446. 112 R 35. 8 : A Incidence and Clinical Significance of k- ras Mutation in the Plasma of Patients with Biliary Carcinoma LIU Hailin, Hiriyuki Watanabe, orio Sawabu ( Dep ar tme nt of Gastroe nter ology , The N i nth Peop l e. s H osp ital, SSM U, Shanghai 2000 11 ) Abstract: Obj ecti ve To study w hether k- ras mutations can be detected in the plasma of pa- tients w ith biliary cancers and ascertain the possibility of developing it as a non - invasive diagnostic marker of biliary cancers clinically. Methods DNA was extracted from plasma, k- ras mutations at codon 12 w ere determined by using the mutant allele specific amplification( MASA) method. Results The incidence of k- ras mutations in gallbladder carcinoma, intrahepatic cholangiocellular carcinoma and common duct cancer w as 5/ ( 1. 4%) , 2/ 6( 33. 3%) , and 1/ 3( 33. 3% ) respectively. k- ras mu- tations w ere not detected in the healthy controls. Concl usion Detection of k- ras mutations in plas- ma is useful for the diagnosis of biliary cancers, especially gallbladder carcinoma. Key words: carcinoma- genetics gene- ras mutant allele specific amplification DNA , k- ras [ 1~ 3] k