骨质疏松症与血清转化生长因子β瘦素的关系.docVIP

骨质疏松症与血清转化生长因子β、瘦素的关系 　　作者：张磊，　　戴冀斌，　　施永彦，　　崔操 　　[摘要]目的：研究骨质疏松症与血清瘦素和转化生长因子Β(TGF-Β)浓度的关系。方法：酶联免疫吸附法(ELISA)检测24例骨质疏松症患者血清中瘦素和TGF-Β水平，并与正常人对照;计算血清瘦素、TGF-Β与骨密度的相关性。结果：骨质疏松症患者TGF-Β水平较对照组明显降低(P0.05)，但血清瘦素与对照组无显著差异(P0.05)。血清TGF-Β与骨密度呈正相关，瘦素与骨密度无相关性。结论：TGF-Β水平降低可能是骨质疏松症发病的原因之一，而无直接证据表明瘦素水平变化与骨质疏松症发病有相关性。 　　[关键词]骨质疏松症;瘦素;转化生长因子Β;酶联免疫吸附检测 　　Relationship Between Osteoporosis and Serum Transforming Growth Factor Β and Leptin 　　ZHANG Lei,DAI Ji-Bin,SHI Yong-yan,CUI Cao. 　　(Faculty of Anatomy and Embryology,School of Medicine,Wuhan University,Wuhan,Hubei 430071;Department of Trauma Orthopaedics,Renmin Hospital,Department of Orthopaedics,Taihe Hospital,Yunyang Medical College,Shiyan,Hubei 442000,China) 　　Abstract: Objective To explore the relationship between osteoporosis and leptin and transforming growth factor Β (TGF-Β) levels. Methods Serum leptin and TGF-Β in 24 patients with osteoporosis were measured by ELISA, and compared with that in 24 healthy subjects as control. Results The serum TGF-Β in the patients with osteoporosis was significantly lower than that in healthy subjects.however,there was no significant difference in serum leptin between patients and healthy subjects.The serum TGF-Β was positively correlated with bone mineral density.However,there is not correlation between serum leptin and bone mineral density. Conclusion The decreased level of TGF-Β may be one of the causes to precipitate osteoporosis, but there is no direct evidence demonstrating the change of serum leptin is correlated with the pathogonesis of osteoporosis. 　　Keywords: Osteoporosis;Leptin;Transforming growth factor Β;ELISA 　　骨质疏松症是一种以全身骨量减少，骨微细结构退化为特征，骨密度降低，致使骨的脆性增加而易于发生骨折的一种全身性骨骼疾病,尤以绝经后妇女为甚。成骨能力下降被认为是骨质疏松症的主要发病机制。转化生长因子Β(transforming growth factor Β, TGF-Β)和瘦素(leptin)对成骨有重要调节作用。本研究通过检测骨质疏松症患者血清瘦素和TGF-Β水平，以探求二者与骨质疏松症发病是否存在相关性，进一步阐明这两种细胞因子在骨质疏松症发病中的作用。 　　1　　材料与方法 　　1.1　　标本采集按照中国人骨质疏松症的建议诊断标准(第二稿)[1]，随机选取骨质疏松症病人24例为骨质疏松组，其中男性6例，女性18例，最大年龄63岁，最小年龄42岁，平均年龄(54