49丹参药材提取工艺的研究.pdfVIP

33 8 2010 8 562 Journal of Beijing Un iversity ofT rad itional ChineseM ed icine Vol. 33 No. 8 Aug. 2010 丹参药材提取工艺的研究* # 玥 杜守颖 ( 北京中医药大学中药学院 北京 100102) : 通过选择 1种溶剂同时提取丹参药材中的脂溶性成分和水溶性成分, 以期能达到节约 成本的 的 采用正交试验设计的方法, 选择乙醇浓度溶剂倍数提取时间和提取次数 4 个因素, 以丹参中丹参酮∀A 和丹酚酸 B 为 2 个指标成分, 考察并确立了丹参的乙醇回流提取工 艺 在实际生产中, 如果侧重于对丹参药材中丹参酮∀A 的提取, 可以采取 6 号工艺, 即 85%乙醇提取, 加水 12倍量, 提取 2次, 每次 2 h; 如果兼顾丹参酮∀A 和丹酚酸 B, 可以采取 2号或 3 号工艺, 即 75%乙醇提取, 加水 10倍量, 提取 2次, 每次 15 h, 或 75%乙醇提取, 加水 12倍量, 提 取 3次, 每次2 h 新工艺可兼顾提取丹参药材的脂溶性和水溶性成分, 且省时省工节能 : 丹参; 丹参酮∀ ; 丹酚酸 B; 提取工艺 A : R283 * Extractive technique ofDanshen (Rad ix Sa lvia eM iltiorrh izae ) # WANG Yue, DU Shouying , MA Yong ( School of Chinese Pharmacy, Beijing University of ChineseMedicine, Beijing 100102) Abstract: Objective To extract the watersoluble and fatsoluble constituents smi ultaneously from Danshen (R ad ix S alv iaeM iltiorrh izae ) through selecting one solvent, and to save the costs. Methods The extractive technique of alcohol reflux of Danshen was exam ined and determ ined by using orthogonal testmethod taking tanshinone ∀ A and salvianolic acid B ( SalB) as indexes after selecting 4 factors, including alcohol concentration ( A), solvent multiple ( B ), extraction tmi e ( C ) and tmi es ( D ). Results If tanshinone ∀A was extracted, the No. 6 technique could be applied: 85% alcoholw ith 12 tmi eswater for 2 hours in 2 tmi es. If tanshinone ∀A and SalB were extracted sy