基于纳米金_多壁碳纳米管_普鲁士蓝_壳聚糖_省略_物_蛋白A定向固定甲胎蛋白免疫.pdfVIP

32 23 Vol. 32, No. 23 2478 2010 12 15 ACTA ACADEM IAE MEDICINAE M ILITARIS TERT IAE Dec. 15 2010 文章编号: 1000-5404( 2010) 23-2478-05 /--/A 1, 2 1 2 1 1 1 龚俊华 , 郑峻松 , 宋明辉 , 李 艳 , 程 平 ( 400038 , ; 066100 2 , ) [] 目的 ( ITO) , --(MWNTs- PB-CH IT ) A( PA)(AFP)方法 ITO , MWNTs-PB-CH IT ITO, ( pH 6. 5), A( ant-iAFP), 结果 , AFP 1. 0~ 250. 0 ng/m l , 0. 5 ng/m l, 6 m in, 3% 结论 [] ; ; --; A; ITO [] R319; R341; R392-33 [] A Direct fixation of alpha-fetoprotein immunosensor based on multi-wall carbon nanotubes-Prussian blue-chitosan nanocomposite and proteinA 1, 2 1 2 1 1 1 Gong Junhua , Zheng Junsong , SongM inghui, LiYan , Cheng Ping ( Departm ent of Clin ical LaboratoryM edicine, College ofM ed ical Laboratory, Th ird M ilitary M ed ical Un iversity, Chongq ing, 400038; 2 Beidaihe Sanatorium of Beijing M ilitary Comm and, Q inhuangdao, H ebeiProvince, 066100, Ch ina) [Abstract] O bjective To construct the highly alpha-fetoprotein (AFP ) sensitive mi munosensor based onmult-iwall carbon nanotubes-prussian blue-chitosan (MWNT s-PB-CH IT ) nanocomposite and proteinA ( PA) using indium tin oxides ( ITO) electrode. M e thods N ano-Auwas electrically deposited on the surface of ITO electrode. MWNT s-PB-CH IT nanocompositewasdropped onto the ITO electrode surfacemodified by gold nanoparticles. PA on the composite surface was adsorbed through the electrostatic and chitosan force w ith negative charge for mi mobilizing AFP antibodies at pH 6. 5. Resu lts The peak current value of the mi mu- nosensorwas linearly relatedw ith theAF