甜瓜蒂中葫芦素B提取工艺研究.pdfVIP

食品科技 2010 年第35 卷第5 期 FOOD SCIENCE AND TECHNOLOGY 提取物与应用 甜瓜蒂中葫芦素B 提取 工艺研究 单海峰， 唐 岚， 李 乐， 单伟光* (浙江工业大学，杭州310014) 摘要： 采用乙醇回流提取的方法筛选了从甜瓜蒂中提取葫芦素B 的最佳工艺。方法：葫芦素B 的含量为评价指标，用正交试验的方法对乙醇浓度、料液比、提取时间、提取次数4 个因素进 行了考察，采用高效液相色谱法测定葫芦素B 的含量：色谱柱为XDB-C18(4.6 mm×150 mm)，流 动相为甲醇-水(60∶40 ，v/v) ，流速为0.8 mL/min ，柱温25 ℃ ，检测波长为228 nm 。结果：得到 最佳工艺条件为：以65% 乙醇为溶剂，在80 ℃条件下，料液比11∶1，回流提取0.5 h ，提取次数 3 次,其葫芦素B 的相对提取率可达86.15%。结论：优选所得工艺合理可行。 关键词： 甜瓜蒂；葫芦素B ；提取工艺 中图分类号： TS 201.2 文献标志码： A 文章编号： 1005-9989(2010)05-0219-03 Study on the extraction process of cucurbitacin B from Cucumis melo L. SHAN Hai-feng, TANG Lan, LI Le, SHAN Wei-guang* (Zhejiang University of Technology, Hangzhou 310014) Abstract: Study on the ethyl alcohol extraction in the cucurbitacin B extraction process from Cucumis melo L. Take in cucurbitacin B content as a target, with orthogonal tests method to the ethyl alcohol density, ratio of material to liquid, the extraction time, times of extraction 4 factors has conducted the research. Uses the HPLC determination: the chromatographic column is XDB-C18 (4.6 mm×150 mm), the flowing for the methyl alcohol - water(60 ∶40, v/v), the speed of flow is 0.8 mL/min, the column warm 25 ℃, the examination wave length is 228 nm. Obtains the best technological conditions is: Under 80 ℃ the condition, take 65% ethyl alcohol as the resolver, ratio of material to liquid 11, the extraction time is 0.5 h, the times of extraction is 3 times, its cucurbitacin B relative extraction rate is 86.15%. The process is feasible, reasonable. Key words: Cucumismelo L.; cucurbitacin B; extraction