黄芪、丹参注射液对乳鼠肥大心肌细胞钙瞬变的影响.pdfVIP

黄芪、丹参注射液对乳鼠肥大心肌 细胞钙瞬变的影响 孙娅楠1李思耐1马淑骅2林谦1 1北京中医药大学东方医院，北京1000782中国中医科学研究院医学实验中心，北京100700 摘要：目的：研究黄芪注射液、丹参注射液对体外培养乳鼠肥大心肌细胞钙瞬变的影响。方法：体外培养乳 鼠心肌细胞，采用血管紧张素Ⅱ(AngⅡ)致心肌细胞肥大，分为对照组、模型组、黄芪组、丹参组、联合组、 洛沙坦组。造模后24、48、72h检测心肌细胞面积与直径，应用激光共聚焦显微镜检测单个心肌细胞钙瞬变。结 果：①各时点模型组心肌细胞核及细胞质面积、直径增加(P＜0.01)；②与对照组相比，24h模型组使钙瞬变荧 光强度峰回落时间增加，48h荧光强度变化率降低；至72h荧光强度亦显著降低；③各治疗组24h峰回落时间显著 降低，48h不仅峰回落时间降低，而且荧光强度变化率增加，72h此效果持续。结论：黄芪注射液、丹参注射液及 其联合应用可调节肥大心肌细胞内钙瞬变异常，从而改善心肌细胞兴奋-收缩偶联。 关键词：黄芪注射液；丹参注射液；心肌肥大；钙瞬变 EffectsofRaidxAstragaliandRadixSalviaeMiltiorrhizaeoncalciumtransientin cardiomyocytehypertrophy SUNYa-nan1LISi-nai1MAShu-hua2LINQian1 1DongfangHospitalofBeijingUniversityofChineseMedicine,Beijing100078,China 2ExperimentalResearchCenterofChina AcademyofChineseMedicalSciences,Bcijing100700,China Abstract:Objective:TostudytheeffectofRaidxAstragaliandRadixSalviaeMiltiorrhizaeoncalciumtransient incardiachypertrophyandthemechanismofRaidxAstragaliandRadixSalviaeMiltiorrhizaeagainstheartfailure.Methods: ThemodelofcardiachypertrophywasinducedbyangiotensinⅡ.Culturedcellsderivedfromneonatalheartwereatrandom dividedinto5groups:controlgroup,modelgroup,Astragaligroup,Salviaegroup,combinationtreatmentgroup.Cardiomyocyte hypertrophywasevaluatedbymeasuringcellareaanddiameter.Usinglaserscanningconfecalmicroscopyscannedthecalcium transientofindividualmyocardialcellsfor24hours,48hoursand72hoursafterthetreatment.Results:AngiotensinⅡdecreased theareaanddiameterofcardiomyocyte.Comparedmodelgroupwithcontrolgroup,theRT50increasedsignificantlyat24hours. At48htheFluo-4ratiodecreasedthan24hours.Until72hoursthemagnitudeofcalciumtransientdecreasedsignificantly.Allthe treatedgroupscoulddecreasedRT50from24hours,andincreasedtheRatiooffluorescenceintensityfrom48hours.Conclusion: RaidxAstragaliandRadixSalviaeMiltiorrhizaeintreatmentcouldregulatethecalciumtransi