高效液相色谱_示差折光检测器测定柴油中多环芳烃.pdfVIP

10 福建分析测试 Fujian Analysis Testing 2010 ，19（4） 高效液相色谱- 示差折光检测器测定柴油中多环芳烃 孙若男，郭武，管嵩，邹雯雯，王静静 （山东出入境检验检疫技术中心，山东 青岛 266500） 摘 要：本文开展了柴油中多环芳烃（PAHs）的高效液相色谱（HPLC）分析方法的研究。氨基柱做固定相，正庚烷为流 动相，采用示差折光检测器进行定性和定量分析。优化了柱流量和反冲洗时间，当柴油样品中双环芳烃（DAHs）加标 + 浓度水平分别为0.8、2.0、4.0g/100mL，三环（及三环以上）芳烃（T AHs）加标浓度水平分别为0.08、0.20、0.40g/100mL 时，加标回收率分别为94%~108%和92%~106%，相对标准偏差分别为0.72%~3.18%及1.14~5.46%。该分析方法已成功 地应用于柴油中PAHs 的分析，其结果令人满意。 关键词：高效液相色谱；示差折光检测器；柴油；多环芳烃 中图分类号：O657.72 文献标识码：A 文章编号：1009- 8143（2010）04- 0010- 03 Determination of PAHs in Diesel by High Performance Liquid Chromatography with Refractive Index Detector Sun Ruo-nan,Guo wu,Guan song,Zou Wen-wen,Wang Jing-jing （Shandong Technical Center of Inspection ＆ Quarantine,Qingdao,Shandong266500,China） Abstract ：An analytical method was developed for the determination of PAHs in diesel by HPLC- RID.The Zorbax NH3 columns was as immobile phase ，and the mobile phase was heptane. Samples were diluted by heptane and then determined by HPLC- RID.Finally,the flow rate of the mobile phase and the backflush were optimized. The recovery studies were performed at + 0.8、2.0、4.0g/100mL fortification levels for DAHs, and 0.08 、0.20、0.40 g/100mL for T AHs, the recoveries ranged from 94% ~108% with a relative standard deviation between 0.72% and 3.18% for DAHs,and 92% ~106% with a relative standard + deviation between 1.14% and 5.46% for T AHs 。 Keywords ：HPLC; RID; diesel; PAHs