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Chinese 5/2012 试验研究
4 Journal
ofRabbitFarming
家兔GHSR基因遗传多态性研究
刘云福,彭瑾,张龚炜,肖正龙,张娟丽,张文秀,赖松家’
(四川农业大学动物科技学院,成都625014)
摘要:GHSR基因编码促生长激素分泌素受体,被认为是生长性状的候选基因,但其在家兔上的研究
甚少。本试验采用PCR产物直接测序法寻找GHSR基因遗传变异,应用HRM分型技术对新西兰兔、加
利福尼亚兔和天府黑兔3个肉兔品种共176个个体进行基因型分析。结果表明:在GHSR基因外显子2
5和0.295
个品种中平均基因频率分别为0.704
关键词:家兔:GHSR:单核苷酸多态性:HRM
中图分类号:S829.1 文献标识码:A 文章编号:1005—6327(2012)05—0004—04
theGenetic ofGHSRGeneinBreeds
Analysis Polymorphism
LIU
Yu¨u,PENGJin,ZHANG Juan-li,ZHANGWen-xiu,LAI
Gong-wei,XlAOZheng-long,ZHANG Song-jia
nimalScienceand A 62501
(CollegeofA Technology,SichuangriculturalUniversity,Chengdu4)
Abstract:GHSRencodethe of hormone usedasa
gene receptorgrowth secretagogue(GHS),waswidely
candidateof its roleindomesticrabbitshasnotbeen
trait,while
genegrowth potential intensivelyinvestigated.
Inthis inexon 1
SNPlociofGHSRGene identifieddirect ofPCR
study,the 2(c.98CT)was by sequencing
1 Zealand
76 fromNew blackrabbitswere
production.Totalsamples rabbits,Californiarabbits,Tianfu
HRM resultsshowedthe ofc.918Cand
genotypingby technology.The averagefrequencies c.918Twere
re 0.7045and0.2955.The ofCC、CTand were0.557.0.295and
spectively frequencies TIgenotypes
0.148.The ofc.918CTinthreerabbitsshowedmoderate
polymorphism
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