副溶血性弧菌生长预测模型的建立与应用探讨.pdfVIP

( ) 华北农学报 ·2 0 0 8 , 2 3 增刊 : 2 6 32 6 7 副溶血性弧菌生长预测模型的建立与应用探讨 王璐华 ,宁喜斌 (上海水产大学 食品学院 ,上海 　200090) 　　摘要 :通过测定 2 株副溶血性弧菌 4～40 ℃的生长曲线 ,研究它们的生长规律 ,运用预测食品微生物学的方法 ,分 别对它们建立初级和二级预测模型 。结果表明 ,副溶血性弧菌的生长曲线在低温阶段 ,适用线性回归方程拟合 ;在适 温阶段 ,生长曲线都呈典型的 S 形 ,适合用 Gompertz 模型拟合 。二级模型采用平方根方程的扩展式 Ratkowsky3 式拟 合 ,根据二级预测模型 ,分别得出副溶血性弧菌的最低生长温度 Tmin ,最高生长温度 Tmax 和最适生长温度 。模型验 证表明 ,预测值和实测值的残差都在 ±005 范围内,偏差因子和准确因子均在可接受范围内,模型是可靠的。最后 , 讨论了模型的应用前景 。 关键词 :预测微生物学 ;模型 ;副溶血性弧菌 ;温度 ( ) 中图分类号 :S432 . 4 　　文献标识码 :A 　　文章编号 :1000 - 7091 2008 增刊 - 0263 - 05 Predictive Model for Effect of Temperature on the Growth of Vibrio Par ahaemolyticus WAN G Luhua ,NIN G Xibin (College of Food Science ,Shanghai Fisheries University ,Shanghai 　200090 ,China) Abstract :The growth characteristics of two Vibrio p arahaemoly ticus ( V. p for short ) stains were studied by using the method of predictive microbiology . The primary and secondary predictive models of Vibrio Parahaemoly ticus were cre ated based on their growth conditions at 4 - 40 ℃. The result showed that the Gompertz model was found to fit with the experimental curves which are typical sigmoidal at fitting temperature and the Monod function were found to fit with the experimental curves at low temperature . The secondary models for the effects of temperature were modeled . Data process ing of the model has shown the minimum growth temperature , the maximum growth temperature and the optimal growth temperature for two V. p stains. Validation of the models showed that the residual difference of predicted and observe