CYP1B1基因缺失小鼠在高糖皮质激素环境下对青光眼的易感性.pdf

·385· ·实验研究· CYPlBl基因缺失小鼠在高糖皮质激素环境下 对青光眼的易感性 吴畅 姜发纲 刘晓雯 ofCYPlB1 to ina knock-outmouse Susceptibility glaucomahigh environment glucocorticOid WU Chang，JIANGFa—gang，LIUXiao—wen．DepartmentofOphthalmology，UnionHospitalof乃n酊Medical Science and College，HuazhongUniversityof Technology，Wuhan430022，China Abstract Geneticfactominvolvesthe of has Background congenital already pathogenesisprimary glaucoma(PCG)．It beenconfirmedthatCYPIBlislocatedin ofthe onPCG—related the GLC3A，onedisease-causinggenes loci．However，whether occurrenceofPCGiscaused theCYPlBImutationsorwithother orenvironmentalfactorremainsunclear． directly by genetic The wasto the of inCYPIBIknock—outmouseunder 0bjective presentstudy investigatesusceptibilityglaucoma gene high of environment．MethodsGlaucomamodels induced of0．04mL dosageglucocorticoid were bysubeonjunetivalinjection 4timesat intervalinthe of40SPF SPFadult betamethasonefor adult C57BL／6Jmice 3-day fighteyes CYPlBI“mice．Forty withthesametreatmentmethodwereascontr01．Theintraocular miceweremeasured XL pressure(10P)of