3-AB对脓毒症大鼠心肌细胞PARP-1、Bcl-2表达的影响.pdf

· ·318 CHINAJOURNALOFEMERGENCYRESUSCITATIONANDDISASTERMEDICINEApril2014Vol.9,No4. ·论著· 3-AB对脓毒症大鼠心肌细胞PARP-1、 Bcl-2表达的影响 王希锋李小丽张毅赵伟刘同慎张连双 【摘要】目的探讨PARP-1 抑制剂3-AB 对脓毒症大鼠心肌细胞PARP-1、Bcl-2 表达的影响。方法 成年健康SD 大鼠30 只，随机分为3 组：假手术对照组、模型组和3-AB 治疗组。采用盲肠结扎穿孔术制作 脓毒症大鼠模型，存活至48h 取材。用免疫组织化学SP 法和图像分析技术检测心肌PARP-1、Bcl-2 的表 达，ELISA 法检测血清中PARP-1、Bcl-2 的含量。结果模型组心肌PARP-1 阳性细胞灰度值、血清中的OD P 0.05 ；而Bcl-2 的变化 值(optical density)均较假手术组升高，而治疗组较模型组下降，差异有统计学意义， 趋势正好相反，模型组心肌Bcl-2 阳性细胞灰度值、血清中的OD 值均较假手术组降低，3-AB 治疗后其水 平较模型组升高，P 0.05。结论PARP-1 抑制剂3-AB 可上调脓毒症大鼠大心肌Bcl-2 的表达，对凋亡有 一定的抑制作用。 【关键词】3-AB ；脓毒症；PARP-1 ；Bcl-2 Theeffectof3-ABonexpressionofPARP-1andBcl-2inmyocardiumofratswithsepsis * * WANG Xi-feng, LI Xiao-li, ZHANG Yi, ZHAO Wei, LIU Tong-shen, ZHANG Lian-shuang. ICU, Yan Tai Yu Huang Ding Hospital; Yantai, Shandong 264000, China Corresponding author: ZHANG Lian-Shuang ，Email: zls197600@126.com 【Abstract】Objective To explore the effect of 3-AB on expression of PARP-1and Bcl-2 in myocardium of rats with sepsis. Methods30 adult health rats were randomly divided into the sham operation group, the model group, and the therapy group with 3-AB. The rats were sacrificed on 48 hours after operation. The expression of PARP-1 and Bcl-2 in myocardium was determined by immunohistochemical staining. The immunepositive gray scale of the immune reactant was detected by computer image analysis technique. The OD (optical density) of PARP-1 and Bcl-2 in serum were detected by ELISA. ResultsIn the model group, the expression of PARP-1 in the myoca