野生黑木耳多糖的提取及性质的研究.pdfVIP

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  • 2015-10-12 发布于江苏
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野生黑木耳多糖的提取及性质的研究.pdf

因此,选用双氧水脱色效果较好。脱色脱蛋白后的多糖,经透析法除去小南子杂 质,经醇沉淀、离心、冷冻干燥得纯多糖。纯多糖经酸水解后制成多糖水解液, 用纸色谱法进行单糖组分分析。结果表明:野生黑木耳多糖由半乳糖和木糖两种 单糖组成,Rf值分别是:O’36和0.44。 5、探讨野生黑木耳多糖(wAAu)对小鼠诱导型高脂血症降脂作用的影响。 选用雄性昆明小鼠70只,经高脂饲料诱导成高脂血症模型,按体重和血清总超 固醇水平均衡分为7组,其中6个实验组分别经胃灌注一定量的WAAU多糖, 每天一次,连续30天,对照组用等剂量的蒸馏水代替,观察WAAU多糖对小鼠 体重,食量的影响及每周测定血浆脂质变化。结果表明:注射量在5%以上时小 鼠的血清总胆固醇(TC)、血清甘油三脂(TG)显著降低(口o.05)和血清高密 度胆固醇(HDL—c)30后有显著升高(pO.05)。当多糖注射量为5%以上时,具 有明显的降脂效果。 关键词:野生黑木耳多糖,提取,纯化,单糖组分分析,理化性质 Abstract nle fiber,VC,VBI, nutrition,includingprotein,fat.totalsugar,water,ash,crude wild and ofthe and Auricularia VB2,Fe,Se,Ca,Zn log-cultivated polysaccharide were and DabieMounraininAthtlUi tested auricula(Hook.1Underw(WAAU)fiom thetraditionalmethodandthe compared,respectively.Both microwave—assisting the methodwereusedtoextract and wasstudied.nleextracted polysaccharide quality was and of werealso polysaccharideseparatedpnrified.Theingredientssinglesugar theeffectsofWAAU the miceto on analyzed.And polysaccharidehyperlipemia decreaselevelwerestudied.1Ihemainresultsobtainedareasfollows. lipid 1 Thesecontentsof and intheWAAUare protein,fat,totalsugarpolysaccharide than 0.7%,O.066%,l。4%and2.96%morethoseof ones. respectively log-cultiva

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