乳鼠角朊细胞分离表达共刺激分子CD80CD86.pdfVIP

http :// Neonatal Murine Keratinocytes Split Express CD80/CD86 upon Culture 1 LeiJianjun ，ChengJingqiu，LiYouping，LiShengfu，ZhangLi ，KeNengwen Key Lab of Transplant Engineering and Immunology MOH,West China Hospital Sichuan University Chengdu, P, R. China 610041 Abstract It was previously thought that keratinocytes did not express the CD80 and CD86 which provide the most important costimulatory signals for the antigen-specific T-cell activation.The cultured keratinocytes allografts were initially accepted,but eventually,allgrafted donor cells were gradually replaced by recipient cells.The precise mechanisms are not very clear.In this study,neonatal murine keratinocytes were cultured for 7 days,the results of flowcytometry and confocal microscopy showed that CD80 could be detected on keratinocytes,whileCD86 could not be detected all the time.RT-PCR analysis confirmed this result.The expression level of the CD80 mRNA amplified significantly from day 1 to day 7,as expression of the control B-actin,but CD86 was not detected.Mixed Lymphocyte Reaction MLR showed that keratinocytes cultured with 10% serum for 7d stimulated effectively allogeneic rather than syngeneic T cell proliferation .This study demonstrated for the first time that co-stimulatory molecule CD80 can be expressed onkeratinocytes invitro.These data provided an alternative explanation for the ultimate rejection of allogeneic keratinocytes in which keratinocytes act as antigen-presenting cells. Keywords: Co-stimulation T cell activation Rejection Keratinocytes 1 Introduction Skin consists of two layers, the epidermis and the dermis. Epidermis have higher immunogenic and easier induce rejection than dermis in transplantation . Epidermal cells consist predominantly of keratinocytes 95–97% and adnexal cells glands, hair, nails , but also include melanocytes,