高温胁迫对茶树叶片光合系统的影响.pdfVIP

茶叶科学 2015，35 （5 ）：415~422 Journal of Tea Science 投稿平台： 高温胁迫对茶树叶片光合系统的影响 * * 李治鑫，李鑫 ，范利超，韩文炎 中国农业科学院茶叶研究所，浙江 杭州 310008 摘要：以龙井43 为材料，高温（43 ℃）处理48 h 后，分别用调制荧光成像系统和双通道荧光仪分析其受胁迫的 状态和光合系统的受损情况。结果表明，受到高温胁迫后：茶树会表现出一系列的热害症状；茶树叶片的光合速 率持续下降，Rubisco 最大羧化速率（Vc,max ）以及RuBP 的最大再生速率（Jmax ）显著降低；Fv/Fm 、Y(II)和Y(I) 迅速降低，Y(NO)和 Y(NA)上升，说明光系统 II 和光系统 I 的结构在高温胁迫下受到了伤害；茶树的 ΦPS Ⅱ、 ETR(II)和 ETR(I)均显著下降，表明电子传递受到严重破坏；光系统 I 对高温胁迫的耐受性较高，抗强光损伤 能力在高温胁迫下变强，而光系统II 易受到高温伤害，抗强光损伤能力在高温胁迫下变弱。 关键词：高温胁迫；茶树；最大光化学量子产量；光系统I ；光系统II 中图分类号：S571.1 ；Q945. 11；P423 文献标识码：A 文章编号：1000-369X （2015 ）05-415-08 Effect of Heat Stress on the Photosynthesis System of Tea Leaves * * LI Zhixin, LI Xin , FAN Lichao, HAN Wenyan Tea Research Institute, Chinese Academy of Agricultural Science s, Hangzhou 310008, China Abastract : To study the effects of heat stress on the photosynthesis system of tea plant (Camellia sinensis L.), seedlings of ‘Longjing 43 ’ cultivar were challenged with high temperature (43 ℃) for 48 h followed by observation of photosynthetic apparatus by using Imaging Fluorescence system and Dual -PAM. Results obtained were as follows: Heat stress caused obvious alterations in the leaf phenotype . Net photosynthetic rate declined gradually fo llowing heat stress which was accompanied with significant decreases in the maximum carboxylation rate of Rubisco and the maximum RuBP regeneration rate . Heat stress decreased Fv/Fm, Y(I) and Y (II) rapidly, but increased Y(NO) and Y (NA), indicating that both photosystem II and ph