原创力文档《Using Genome-Referenced Expressed Sequence Tag Assembly to Analyze the Origin and Expression Patterns of Gossypium hirsutum Transcripts》.pdfVIP
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《Using Genome-Referenced Expressed Sequence Tag Assembly to Analyze the Origin and Expression Patterns of Gossypium hirsutum Transcripts》.pdf
Journalof Plant 201
IntegrativeBiology3，55(7)：576—585
Genome--Referenced
Using ExpressedSequenceTag
to the and
AssemblyAnalyzeOriginExpression
Patternsof hirsutumTranscri
Gossypium pts回
Jinl，Qin Xia01andYu．XianZhul，2术
Xiang Lil，Guanghui
’State ofProteinandPlantGene ofLife 100871，China
KeyLaboratory Research，CollegeSciences，PekingUniversity，Beijing
2NationalCenterforPlantGene 1001
Research(Beijing)．Beijing01，China
author
+Corresponding
Tel：+861062751 6275
193；Fax：+864427；E—mail：zhuyx2@pku．edu．ca
II=1Articlescanbeviewedonlinewithoutasubscription．
Availableonlineon15 2013at and
www．wileyonlinelibrary．com／journal／jipb
May www．jipb．net
doi：10．11114ipb．12066
Abstract
weassembledatotaIof
297，239 hirsutum(Gh，a
Gossypium tetraploid sequence
thatwereavailableintheNationaICenterfor Information
tag(EST)sequences BiotechnoIogvdatabase，
withreferencetothe G．raimondii(Gr，a obtained
recentlypublished diploidcotton，DD)genome，and
49．125UniGenes。The oftheUniGeneswereincreasedfrom804and791intwo
averageIengths bp previous
ESTassembliesto1．019 inthecurrent numberof cottonUniGeneswith of
bp analysis．Theputative Iengths
3kbormoreincreasedfrom25or34to a of G．hirsutum
1．223．AsresuIt．thousands
ori
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