《响应面法优化超临界CO2提取茶籽多糖的工艺研究 Optimization of Supercritical CO2 Extraction Process of the Polysaccharide from Tea Seed by Response Surface Methodology》.pdfVIP

第24卷第5期 高校化学工程学报 NO．5、，01．24 2010年lO月 JournalofChemical ofChineseUniversities Oct． 20lO Engineering 文章编号：1003-9015 2010 05．0897-06 响应面法优化超临界C02提取茶籽多糖的工艺研究 李博，屠幼英，梅鑫，蔡晓红，王奕，李伟 浙江大学茶学系，浙江杭州310029 摘要：茶多糖具有多种重要的生物活性。本研究采用响应面法优化茶籽多糖的超I|缶界c02提取工艺，运用小星点设 计考察萃取时间 30～150min 、压力 15,--45MPa 、温度 40～80℃ 及夹带剂乙醇浓度 50％一90％ 四因素对茶籽多糖得率 的影响。响应面分析表明，茶籽多糖得率与四因素关系符合二次模型。时间和夹带剂乙醇浓度的一次项和二次项、压力 的二次项以及时问和压力的交互作用对茶杼多糖得率具有显著影响。该二次多项式模型用后退法进行逐步回归得到简化 模型，依据简化后的模型在各因素设定范围内获得最佳提取工艺为：时间150min，压力45MPa，温度60℃，夹带剂 乙醇浓度为65％。该条件下进行三次重复试验，茶耔多糖的实际平均得率为13．23％，与预测值13．96％无显著差异。 关键词：超l|缶界C02；茶籽：多糖；响应面分析；小星点设计 中图分类号：TQ028．32 文献标识码：A of ExtractionProcessofthe fromTea C02 OptimizationSupercritical Polysaceharide Seed Surface by Methodology Response Wei LI Yi，LI Bo。TUYou-ying，MEIXin，CAIXiao—hong，WANG ofTea 3 University，Hangzhou10029，China DepartmentScience，Zhcjiang hasvarious activities．The extraction Abstract：Tea C02 polysaccharideimportantbiological supercritical withtheethanol solutionassolventmodifierwasusedtoextractthe fromtea process aqueous polysaccharide seedandthe surface usedto the process responsemethodology RSM wasoptimizeprocessparameters．Four ethanol time 30-150 the parameters，extractionmin ，pressure 15--45 contentinmodifier selectedasfour variables．Asinailcentral design f500／0--90％ were independent composite extracted theeffectsoftheabovefour variablesonthe ofthe CCDlwasusedto yield small study independent teaseed formulawasobtained surface betweenthe of polysaccharide．Thequadratic byresponseanalysis yield theextractedteaseed andthefour linearand termoftimeandethanol polysaccharideparameters．Thequadratic timeand have termof andtheinteractionbetween concentration,the pressuresignificant quadratic pressure modelwas ified a effectsonthe ofteaseed second-order byusing polynomial simpl yield polysaccharide．The the determined thenthe within were backward method，andoptimumparameters experimentalranges stepwise solution．Underthese tobeatl50 with65％ethanol conditions，theave