《CD14基因-159CT位点多态性对哮喘患者血浆IL-5的影响》.pdfVIP

59 CDl4基因一1 C／T位点多态性对哮喘患者 血浆IL一5的影响 陈敏，吴斌。李文 (广东医学院附属医院，广东湛江524001) 【摘要】 响。方法选择正常对照组和哮喘组各150例，取外周血离心后，用酶联免疫吸附试验法测定血浆1I．,-5，用限制性 片段长度多态性一聚合酶链反应方法检测CDl4基因一159C／T位点多态性分布。结果对照组和哮喘组等位基 位点多态性与哮喘相关，c等位基因与血浆IL-5升高相关。 [关键词】哮喘；CDl4基因；多态性；白细胞介素5 [中图分类号】 R562．2[文献标识码】 A [文章编号】 1002-206X(2009)05-0013-03 on in with InfluenceofCDl4 59C／T lL巧level asthma gene一1 polymorphjsm patients CHEN M／n。WUBin．LI’％n Medical (AffiliatedHospitalofGuangdongCollege，Zhanjian524023，P．R．China) To the betweenthe at一159C／TlocusinCDl4 and correlation Abstract：Objectiveinv∞tigate polymorpMsm gene its IL-5 control asthmaand level．Methods150asthma and150 were imp∞tuponplasm subjects subjectschosen，after reason bloodWq IL-5levelweretested ehmn and peripheral centrifuged，plasm byELISA．Polymerase restriction自Bg- ment wereusedtodetermine一159C／Tlocus The lengtll polymorpMsm．Results polymo印Msm(RFLP·PCR)techniques of T allele distributionCandalleleswel-e differemintheathmaandcontrol significantly group吖=10．82，Po．01)，C 嘲associatedwith and asthma[OR(C／T)=1．73，95％CI=1．25—2．39，P0．01]．Thegenetype(IT，CTCC) in withCallele distributionWasalso differentthetwo each significantly group，subjects