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B1 polydonal antibody and its potential application in non-small cell lung cancer.pdf
维普资讯
hinese—German ournatofClinicalOncology May2008,Vo1.7,No.5,.P249-P25
OI 10.1007/s10330—008—0o23—1
Thepreparationofanti—hnRNPA2/B1polydonaI
antibodyanditspotentialaPPlicationinnon-small
celllungcancer*
LejieCao,YeshanLi,MeiqingXu,RunshengLi。,ZubaoLei,XianwuLi
DepartmentofRespiratoryMedicine,theAffiliatedProvincialHospitalofAnhuiMedicalUniversity,Hefei230001,
China
DepartmentofChestSurgery,theAffiliatedProvincialHospitalofAnhuiMedicalUniversity,Hefei230001,China
。DepartmentofRespiratory Medicine,W uxiFourthPeople’sHospital,W uxi214000,China
DepartmentofRespiratory Medicine,HefeiSecondPeople’sHospital,Hefei230001,China
DepartmentofBioengineering,UniversityofW ashington,Seattle98105,USA
Received:15February2008/Revised:7March2008/Accepted:2April2008
Abstract Objec~ve:lnorderloevaluatepotentialapplicationfordiagnosisandprognosisofnon.smallcel11ungacflcer
(NSCLC),aswellastodetermineitsroleinlhepathogenesisofthedisease,wepreparedanti.humanhnRNPA2JB1potyclonaI
antibody.Me纳ods:ProkaryoticexpressionvectorofpET28a(+).hnRNPA2/B1wasconstructedandbansformedintoE.coli
8L21.TherecombinanlproteinjnducedbyJPTGwaspurifiedandinjectedlorabbitsforantibodypreparation.Expressionof
hnRNPA2/B1wasexaminedin45lissuesofNSCLCand16inflammatorypseudotumorlissuesoflungbyimmunohistochem.
istrywithlheantibody.ThecommercialhnRNPA2/B1monoclonalantibodywasusedasacontro1.-Results:f1)Polyclonalan.
tibodyagainsthnRNPA2/B1withhighlitlewasobtained.(2)Thepositivestaining{nNSCLClissueswas62.22%,whichwas
substantiallyhigherlhanlhalinnormallissues(40%,P=0.035)orinflammatorypseudotumorlissues(3125%,P=0.033).
(3)ExpressionofhnRNPA2/B1positivelycorrelatedwithageandlhehistoryofsmoking,whereasitnegativelycorrelated
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