Molecular Characterization of Olive Cultivars in Iraq Using SSR Markers and Compare with Phenotypic Characterization.pdfVIP

N蠕JournalofLifeSciences6(2012)1343—1350 ’ MolecularCharacterizationof0liveCultivarsinIraq UsingSSR MarkersandComparewithPhenotypic Characterization IqbalHarbi，SalwaJaberA1一AwadiandAliImadM ohammadMoner ． StateBoardofDatePalmMinistryofAgriculture,Baghdad10069,lraq 2．AINahrainForensicDNATrainingCenter,UniversityofAlNahrien，Baghdad10072，Iraq 3．GeneticEngineenngandBiotechnologyInstituteforPostgraduateStudies,BaghdadUniversiyt,Baghdad10071．Iraq Received：June05，2012／Accepted：August14，2012／Published：December30，2012 Abstract：Simplesequencerepeat(SSR)analysiswasusedtostudythegenotyperelationamongtendifferentolivesvarietiesfrom AI—ZarfaniaandA1一Moselstationministryofagriculture／IraqShami，Sorani，Manzenllo，Qaysi，Arbqween，Jlot(Labeeb)，Baashiqi， Dahkan，Nepali，Khodeiri，Fifteen SSR lociweresutdiedandproduced239amplifiedfragment．Twohundredandthirtysevenof theseloci(99．16％)werepolymorphicoverallthegenotypestested．Dendrogram andmatrixofsimilaritywereobtainedbythe UnweightedPair-GroupMethodanalysis(UPGMA)．Sutdyshowedtwogroups：groupA：Nepali，Arbqween，andgroupB：dividedin twosubgroups(subgroupB1：Jlot，Dahkan，subgroupB2：othercultivar)，whilethegenotyperelationaccordingtophenotypewas confused．SSR hasabettermolecularmarkerthanothermoleculartechniquefordetectinggeneticrelationshipamongcultivars，and helpiuknownthepedigreeofrelativesandancestors． Keyword：Olive，PCR，SSR，molecularmarkers 1．Introduction phenology [3]．ButDNA—based markersare not influenced by environmental conditions，and they Olive(OleaeuropaeaL．)isoneofthemostancient allow directscanningoftheplantgenome[4，5]． cultivatedfruittreespeciesintheM editerraneanbasin Knowledgeofthegeneticrelationsh