Molecular level investigation of exocytosis in cultured human pheochromocytoma cells： insights from high resolution scanning electron microscopy combined with autoradiogram and cytochemistry.pdfVIP

Ch in ese Medical Jour na l 1998 ; 111( 4) : 295-301 ·2 95 · Molecular level investigat ion of exocytosis in cultured human pheochr omocytoma cells : in sight s fr om h igh resolution scanning electr on micr oscopy comb ined with autor adiogr am and cytochemistr y Lee Ying- Sh iung 李英雄 and Ch ou Yun- Ying 周云英 　　Obj ec t iv e 　To investigat e molecula r e vents of Based on transmission electron microscopic studies , exocytosis in cultured human phe ochromocytoma ce lls using both th in- sectioned an d freeze- fractured tech- with stimulat ion . niques , th e consecutive stages of t he exocytotic chain re- Met h od s 　The cultured pheochromocytoma ce lls actions of t he secretory granule and cell membranes , in- prep ared from human adrenal phe ochromocytoma tu- cluding t he formation of th e membrane dimples , mem- mor were stimula ted for the re lea se of cate cholamine s brane fusion and pore formation , pore growth and gran- ule disassemb ly have been extensively described in a by de pola riza tion with the adm inistrat ion of 50 mmol/ L 1, 2 large variety of cells . 　The ob servation s from quick KCl . Tra nsmission electron m icro scopy ( T M) and freeze fracture electron microscoopy provided further un- high re solution scann ing e lec tron m icro scopy derstan ding of t he mec